Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/48148
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Almeida, Raquel Costa | por |
dc.contributor.author | Calejo, Isabel | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Gomes, Manuela E. | por |
dc.date.accessioned | 2017-12-11T13:16:21Z | - |
dc.date.issued | 2018 | - |
dc.date.submitted | 2017-12 | - |
dc.identifier.citation | Costa-Almeida R., Calejo I., Reis R. L., Gomes M. E. Crosstalk between adipose stem cells and tendon cells reveals a temporal regulation of tenogenesis by matrix deposition and remodeling, Journal Of Cellular Physiology, doi:10.1002/jcp.26363, 2017 | por |
dc.identifier.issn | 0021-9541 | por |
dc.identifier.uri | https://hdl.handle.net/1822/48148 | - |
dc.description.abstract | Tendon injuries constitute an unmet clinical challenge owing to the limited intrinsic regenerative ability of this tissue. Cell-based therapies aim at improving tendon healing through the delicate orchestration of tissue rebuilding and regain of function. Hence, human adipose-derived stem cells (hASCs) have been proposed as a promising cell source for boosting tendon regeneration. In this work, we investigated the influence of hASCs on native human tendon-derived cells (hTDCs) through the establishment of a direct contact co-culture system. Results demonstrated that direct interactions between these cell types resulted in controlled proliferation and spontaneous cell elongation. ECM-related genes, particularly COL1A1 and TNC, and genes involved in ECM remodeling, such as MMP1, MMP2, MMP3 and TIMP1, were expressed in co-cultures in a temporally regulated manner. In addition, deposition of collagen type I was accelerated in co-cultures systems and favored over the production of collagen type III, resulting in an enhanced COL1/COL3 ratio as soon as 7 days. In conclusion, hASCs seem to be good candidates in modulating the behavior of native tendon cells, particularly through a balanced process of ECM synthesis and degradation. | por |
dc.description.sponsorship | Contract grant sponsor: FCT (Fundação para a Ciência e a Tecnologia, Portugal); Contract grant number: PhD grant SFRH/BD/96593/2013 (R.C-A); consolidator grant IF/00593/2015 (M.E.G); PhD grant PD/BD/128088/2016 (IC) Contract grant sponsor: European Commission, European Regional Development Fund (ERDF); Contract grant number: project RL3-TECT-NORTE-07-0124-FEDER- 000020 cofinanced by ON.2 under the National Strategic Reference Framework (NSRF) | por |
dc.language.iso | eng | por |
dc.publisher | Wiley | por |
dc.relation | SFRH/BD/96593/2013 | por |
dc.relation | IF/00593/2015 | por |
dc.relation | PD/BD/128088/2016 | por |
dc.relation | RL3-TECT-NORTE-07-0124-FEDER-000020 | por |
dc.rights | restrictedAccess | por |
dc.subject | Adipose-derived stem cells | por |
dc.subject | Co-culture | por |
dc.subject | Extracellular matrix | por |
dc.subject | Matrix metalloproteinases | por |
dc.subject | Temporal gene regulation | por |
dc.subject | Tendon Regeneration | por |
dc.subject | Tenogenesis | por |
dc.title | Crosstalk between adipose stem cells and tendon cells reveals a temporal regulation of tenogenesis by matrix deposition and remodeling | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/jcp.26363/full | por |
dc.comments | http://3bs.uminho.pt/node/19266 | por |
oaire.citationStartPage | 5383 | por |
oaire.citationEndPage | 5395 | por |
oaire.citationIssue | 7 | por |
oaire.citationVolume | 233 | por |
dc.date.updated | 2017-12-11T12:01:12Z | - |
dc.identifier.eissn | 1097-4652 | por |
dc.identifier.doi | 10.1002/jcp.26363 | por |
dc.identifier.pmid | 29215729 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Cellular Physiology | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19266-Costa-Almeida_et_al-2017-Journal_of_Cellular_Physiology.pdf Acesso restrito! | 2,15 MB | Adobe PDF | Ver/Abrir |