Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/50943
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Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Carneiro, Maria Catarina Santos | por |
dc.contributor.author | Vaz, Catarina Oliveira | por |
dc.contributor.author | Pais, Célia | por |
dc.contributor.author | Sampaio, Paula | por |
dc.contributor.author | Pereira, Joana Isabel Carvalho | por |
dc.date.accessioned | 2018-02-23T10:14:47Z | - |
dc.date.available | 2018-02-23T10:14:47Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0167-7012 | por |
dc.identifier.uri | https://hdl.handle.net/1822/50943 | - |
dc.description.abstract | Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells. (C) 2014 Elsevier B.V. All rights reserved. | por |
dc.description.sponsorship | This research was supported by FEDER Funds through the Operational Programme COMPETE and national funds through the Fundação para a Ciência e Tecnologia (FCT), in the scope of project PEst-OE/BIA/ UI4050/2014. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/135919/PT | por |
dc.rights | openAccess | por |
dc.subject | Candida spp | por |
dc.subject | Flow cytometry | por |
dc.subject | Sytox green | por |
dc.subject | Propidium iodide | por |
dc.subject | Phagocytosis | por |
dc.subject | PAMPs | por |
dc.title | A new method for yeast phagocytosis analysis by flow cytometry | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
oaire.citationStartPage | 56 | por |
oaire.citationEndPage | 62 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 101 | por |
dc.date.updated | 2018-02-23T09:44:59Z | - |
dc.identifier.doi | 10.1016/j.mimet.2014.03.013 | por |
dc.identifier.pmid | 24704495 | - |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | - |
sdum.export.identifier | 2929 | - |
sdum.journal | Journal of Microbiological Methods | por |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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(2014) J Microbiol Methods .pdf | 1,48 MB | Adobe PDF | Ver/Abrir |