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https://hdl.handle.net/1822/51422
Título: | Nature-derived epigallocatechin gallate/duck’s feet collagen/hydroxyapatite composite sponges for enhanced bone tissue regeneration |
Autor(es): | Kook, Yeon Ji Tian, Jingwen Jeon, Yoo Shin Choi, Min Jung Song, Jeong Eun Park, Chan Hum Reis, R. L. Khang, Gilson |
Palavras-chave: | Bone marrow stromal cells (BMSCs) Bone regeneration Ducks feet collagen (DC) Epigallocatechin gallate (EGCG) Hydroxyapatite (HAp) |
Data: | 2018 |
Editora: | Taylor & Francis |
Revista: | Journal of Biomaterials Science. Polymer Edition |
Citação: | Kook Y. J., Tian J., Jeon Y. S., Choi M. J., Song J. E., Park C. H., Reis R. L., Khang G. Nature-derived epigallocatechin gallate/duck’s feet collagen/hydroxyapatite composite sponges for enhanced bone tissue regeneration, Journal Of Biomaterials Science-polymer Edition, Vol. -, Issue -, pp. 1-13, doi:10.1080/09205063.2017.1414480, 2018 |
Resumo(s): | Scaffolds mimicking structural and chemical characteristics of the native bone tissues are critical for bone tissue engineering. Herein, we have developed and characterized epigallocatechin gallate/duck's feet collagen/hydroxyapatite (EGCG/DC/HAp) composite sponges that enhanced the bone tissue regeneration. The three-dimensional composite sponges were synthesized by loading various amounts (i.e. 1, 5 and 10 μM) of EGCG to duck feet derived collagen followed by freeze-drying and then coating with hydroxyapatite. Several measuremental techniques were employed to examine the properties of the as-fabricated composite sponges including morphology and structure, porosity, compressive strength, etc. and as well compared with pristine duck feet derived collagen. SEM observations of EGCG/DC/HAp sponges showed the formation of a highly porous collagen matrix with EGCG embodiment. The porosity and pore size of sponges were found to increase by high EGCG content. The compressive strength was calculated as 3.54 ± 0.04, 3.63 ± 0.03, 3.89 ± 0.05, 4.047 ± 0.05 MPa for 1, 5 and 10 μM EGCG/DC/HAp sponges, respectively. Osteoblast-like cell (BMSCs isolated from rabbit) culture and in vivo experiments with EGCG/DC/HAp sponges implanted in nude mouse followed by histological staining showed enhanced cell internalization and attachment, cell proliferation, alkaline phosphatase expressions, indicating that EGCG/DC/HAp sponges have ahigh biocompatibility. Moreover, highEGCG content in the EGCG/DC/HAp sponges have led to increased cellular behavior. Collectively, the 5 μM of EGCG/DC/HAp sponges were suggested as the potential candidates for bone tissue regeneration. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/51422 |
DOI: | 10.1080/09205063.2017.1414480 |
ISSN: | 0920-5063 |
e-ISSN: | 1568-5624 |
Versão da editora: | http://www.tandfonline.com/doi/full/10.1080/09205063.2017.1414480 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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GKhang-JBiomSciePolEd2017.pdf | 4,93 MB | Adobe PDF | Ver/Abrir |