Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/53722
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Campo DC | Valor | Idioma |
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dc.contributor.author | Pereira, Diana R. | por |
dc.contributor.author | Silva-Correia, Joana | por |
dc.contributor.author | Oliveira, Joaquim M. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Pandit, Abhay | por |
dc.contributor.author | Biggs, Manus J. | por |
dc.date.accessioned | 2018-03-29T10:50:29Z | - |
dc.date.issued | 2018-04 | - |
dc.identifier.issn | 1549-9634 | por |
dc.identifier.uri | https://hdl.handle.net/1822/53722 | - |
dc.description.abstract | Intervertebral disc (IVD) degeneration is associated with both structural damage and aging related degeneration. Annulus fibrosus (AF) defects such as annular tears, herniation and discectomy require novel tissue engineering strategies to functionally repair AF tissue. An ideal construct will repair the AF by providing physical and biological support, facilitating regeneration. The presented strategy herein proposes a gellan gum-based construct reinforced with cellulose nanocrystals (nCell) as a biological self-gelling AF substitute. Nanocomposite hydrogels were fabricated and characterized with respect to hydrogel swelling capacity, degradation rate in vitro and mechanical properties. Rheological evaluation on the nanocomposites demonstrated the GGMA reinforcement with nCell promoted matrix entanglement with higher scaffold stiffness observed upon ionic crosslinking. Compressive mechanical tests demonstrated compressive modulus values close to those of the human AF tissue. Furthermore, cell culture studies with encapsulated bovine AF cells indicated that nanocomposite constructs promoted cell viability and a physiologically relevant cell morphology for up to fourteen days in vitro. | por |
dc.description.sponsorship | DR Pereira acknowledges the Foundation for Science and Technology (FCT), Portugal, for individual grant SFRH/BD/81356/2011 and also the European Union funding under the 7th Framework Programme under Grant Agreement Number 317304 and supported in part by a research grant from Science Foundation Ireland (SFI) and co-funded under the European Regional Development Fund under Grant Number 13/RC/2073. J.M. Oliveira also thanks the FCT for the funds provided under the program Investigator FTC 2012 and 2015 (IF/00423/2012 and IF/01285/2015). MJ Biggs is a Science Foundation Ireland, Starting Investigator SIRG COFUND fellow (grant no. 11/SIRG/B2135). In addition, MJ Biggs is a funded investigator through the CÚRAM Science Foundation Ireland (grant agreement no. 13/RC/2073). The authors would like to acknowledge the Centre for Microscopy & Imaging funded by National University of Ireland Galway and MSSI in University of Limerick for the rheometer facilities, both funded by the Programme for Research in Third-Level Institutions (PRTLI), Cycles 4 and 5, National Development Plan 2007–2013. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F81356%2F2011/PT | por |
dc.rights | restrictedAccess | por |
dc.subject | Annulus fibrosus | por |
dc.subject | Intervertebral disc degeneration | por |
dc.subject | Methacrylated gellan-gum | por |
dc.subject | Nanocellulose | por |
dc.subject | Nanocomposite hydrogels | por |
dc.subject | Self-gelling | por |
dc.title | Nanocellulose reinforced gellan-gum hydrogels as potential biological substitutes for annulus fibrosus tissue regeneration | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
oaire.citationStartPage | 897 | por |
oaire.citationEndPage | 908 | por |
oaire.citationIssue | 3 | por |
oaire.citationVolume | 14 | por |
dc.date.updated | 2018-03-28T23:19:35Z | - |
dc.identifier.doi | 10.1016/j.nano.2017.11.011 | por |
dc.identifier.pmid | 29170112 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.export.identifier | 4956 | - |
sdum.journal | Nanomedicine: Nanotechnology, Biology, and Medicine | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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PIIS154996341730206X.pdf Acesso restrito! | 2,72 MB | Adobe PDF | Ver/Abrir |