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https://hdl.handle.net/1822/5680
Título: | Functional purification of the monocarboxylate transporter of the yeast Candida utilis |
Autor(es): | Baltazar, Fátima Cássio, Fernanda Leão, Cecília |
Palavras-chave: | Candida utilis Functional reconstitution Lactate carrier Protein purification |
Data: | 2006 |
Editora: | Springer |
Revista: | Biotechnology Letters |
Citação: | "Biotechnol lett". ISSN 0141-5492. 28:16 (Aug. 2006) 1221-1226. |
Resumo(s): | Plasma membranes of the yeast, Candida utilis, were solubilized with octyl-b-D- lucopyranoside and a fraction enriched in the lactate carrier was obtained with DEAE-Sepharose anion- exchange chromatography, after elution with 0.4 M NaCl. The uptake of lactic acid into proteoliposomes, containing the purified protein fraction and cytochrome c oxidase, was dependent on a proton-motive force and the transport specificity was consistent with the one of C. utilis intact cells. Overall, we have obtained a plasma membrane fraction enriched in the lactate carrier of C. utilis in which the transport properties were preserved. Given the similarities between the lactate transport of C. utilis and the one of mammalian cells, this purified system could be further explored to screen for specific lactate inhibitors, with potential therapeutic applications. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/5680 |
DOI: | 10.1007/s10529-006-9088-5 |
ISSN: | 0141-5492 |
Versão da editora: | 0141-5492 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Baltazar - Biotech Lett 2006.pdf | 185,41 kB | Adobe PDF | Ver/Abrir |