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https://hdl.handle.net/1822/57967
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Campo DC | Valor | Idioma |
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dc.contributor.author | Ferreira, Maria João | por |
dc.contributor.author | Pires-Luís, Ana Sílvia | por |
dc.contributor.author | Vieira-Coimbra, Márcia | por |
dc.contributor.author | Costa-Pinheiro, Pedro | por |
dc.contributor.author | Antunes, Luís | por |
dc.contributor.author | Dias, Paula C. | por |
dc.contributor.author | Lobo, Francisco | por |
dc.contributor.author | Oliveira, Jorge | por |
dc.contributor.author | Gonçalves, Celine Saraiva | por |
dc.contributor.author | Costa, Bruno Marques | por |
dc.contributor.author | Henrique, Rui | por |
dc.contributor.author | Jerónimo, Carmen | por |
dc.date.accessioned | 2019-01-09T15:08:54Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Ferreira, M. J., Pires-Luís, A. S., Vieira-Coimbra, M., et. al. (2017). SETDB2 and RIOX2 are differentially expressed among renal cell tumor subtypes, associating with prognosis and metastization. Epigenetics, 12(12), 1057-1064 | por |
dc.identifier.issn | 1559-2294 | - |
dc.identifier.uri | https://hdl.handle.net/1822/57967 | - |
dc.description.abstract | Increasing detection of small renal masses by imaging techniques entails the need for accurate discrimination between benign and malignant renal cell tumors (RCTs) as well as among malignant RCTs, owing to differential risk of progression through metastization. Although histone methylation has been implicated in renal tumorigenesis, its potential as biomarker for renal cell carcinoma (RCC) progression remains largely unexplored. Thus, we aimed to characterize the differential expression of histone methyltransferases (HMTs) and histone demethylases (HDMs) in RCTs to assess their potential as metastasis biomarkers. We found that SETDB2 and RIOX2 (encoding for an HMT and an HDM, respectively) expression levels was significantly altered in RCTs; these genes were further selected for validation by quantitative RT-PCR in 160 RCTs. Moreover, SETDB2, RIOX2, and three genes encoding for enzymes involved in histone methylation (NO66, SETD3, and SMYD2), previously reported by our group, were quantified (RT-PCR) in an independent series of 62 clear cell renal cell carcinoma (ccRCC) to assess its potential role in ccRCC metastasis development. Additional validation was performed using TCGA dataset. SETDB2 and RIOX2 transcripts were overexpressed in RCTs compared to renal normal tissues (RNTs) and in oncocytomas vs. RCCs, with ccRCC and papillary renal cell carcinoma (pRCC) displaying the lowest levels. Low SETDB2 expression levels and higher stage independently predicted shorter disease-free survival. In our 62 ccRCC cohort, significantly higher RIOX2, but not SETDB2, expression levels were depicted in cases that developed metastasis during follow-up. These findings were not apparent in TCGA dataset. We concluded that SETDB2 and RIOX2 might be involved in renal tumorigenesis and RCC progression, especially in metastatic spread. Moreover, SETDB2 expression levels might independently discriminate among RCC subgroups with distinct outcome, whereas higher RIOX2 transcript levels might identify ccRCC cases with more propensity to endure metastatic dissemination. | por |
dc.description.sponsorship | This study was funded by research grants from Research Center of Portuguese Oncology Institute - Porto (CI-IPOP 4-2012 and CI-IPOP 27) and from Associacao Portuguesa de Urologia (APU-2010). ASP-L was supported by FCT-Fundacao para a Ciencia e a Tecnologia fellowship (SFRH/SINTD/94217/2013). CSG is supported by FCT- Fundacao para a Ciencia e Tecnologia PhD fellowships (SFRH/BD/92786/2013) and BMC is funded by FCT-Fundacao para a Ciencia e a Tecnologia (IF/00601/2012). | por |
dc.language.iso | eng | por |
dc.publisher | Taylor and Francis | por |
dc.rights | openAccess | - |
dc.subject | Biomarkers, Tumor | por |
dc.subject | Carcinoma, Renal Cell | por |
dc.subject | Chromosomal Proteins, Non-Histone | por |
dc.subject | Disease-Free Survival | por |
dc.subject | Female | por |
dc.subject | Histone Demethylases | por |
dc.subject | Histone-Lysine N-Methyltransferase | por |
dc.subject | Humans | por |
dc.subject | Kidney Neoplasms | por |
dc.subject | Male | por |
dc.subject | Neoplasm Metastasis | por |
dc.subject | Nuclear Proteins | por |
dc.subject | RNA | por |
dc.subject | biomarker | por |
dc.subject | histone methyltransferase | por |
dc.subject | kidney cancer | por |
dc.subject | metastasis | por |
dc.subject | prognosis | por |
dc.subject | renal cell tumor | por |
dc.subject | renal cell carcinoma | por |
dc.subject | RIOX2 | por |
dc.subject | SETDB2 | por |
dc.title | SETDB2 and RIOX2 are differentially expressed among renal cell tumor subtypes, associating with prognosis and metastization | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.tandfonline.com/doi/abs/10.1080/15592294.2017.1385685 | por |
dc.comments | author can archive post-print (ie final draft post-refereeing) | por |
oaire.citationStartPage | 1057 | por |
oaire.citationEndPage | 1064 | por |
oaire.citationIssue | 12 | por |
oaire.citationVolume | 12 | por |
dc.identifier.eissn | 1559-2308 | - |
dc.identifier.doi | 10.1080/15592294.2017.1385685 | por |
dc.identifier.pmid | 29099276 | por |
dc.subject.fos | Ciências Médicas::Medicina Básica | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Epigenetics | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Ferreira MJ SETDB2 and RIOX2 are differentially expressed among renal cell tumor subtypes associating.pdf | 891,08 kB | Adobe PDF | Ver/Abrir |