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https://hdl.handle.net/1822/58255
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Campo DC | Valor | Idioma |
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dc.contributor.author | Rogeri, Caroline Domingues | por |
dc.contributor.author | Silveira, Henrique César Santejo | por |
dc.contributor.author | Causin, Rhafaela Lima | por |
dc.contributor.author | Villa, Luisa Lina | por |
dc.contributor.author | Stein, Maíra Degiovani | por |
dc.contributor.author | de Carvalho, Ana Carolina | por |
dc.contributor.author | Arantes, Lídia Maria Rebolho Batista | por |
dc.contributor.author | Scapulatempo-Neto, Cristovam | por |
dc.contributor.author | Possati-Resende, Júlio César | por |
dc.contributor.author | Antoniazzi, Márcio | por |
dc.contributor.author | Longatto, Adhemar | por |
dc.contributor.author | Fregnani, José Humberto Tavares Guerreiro | por |
dc.date.accessioned | 2019-01-16T10:03:57Z | - |
dc.date.issued | 2018-09 | - |
dc.identifier.citation | Rogeri, C. D., Silveira, H. C. S., Causin, R. L., Villa, L. L., Stein, M. D., de Carvalho, A. C., ... & Longatto-Filho, A. (2018). Methylation of the hsa-miR-124, SOX1, TERT, and LMX1A genes as biomarkers for precursor lesions in cervical cancer. Gynecologic oncology, 150(3), 545-551 | por |
dc.identifier.issn | 0090-8258 | - |
dc.identifier.uri | https://hdl.handle.net/1822/58255 | - |
dc.description.abstract | The methylation profile of genes in precursor lesions in cervical cancer was characterized to improve screening techniques for high-grade intraepithelial neoplasia. | por |
dc.description.abstract | Objectives The methylation profile of genes in precursor lesions in cervical cancer was characterized to improve screening techniques for high-grade intraepithelial neoplasia. Methods A total of 447 cervical cytology samples obtained from women who underwent colposcopy were examined. The cases were distributed as follows: (1) cervices without cervical intraepithelial neoplasia (CIN; n = 152); (2) cervices with a CIN grade of 1 (CIN 1; n = 147); and (3) cervices with a CIN grade of 2 or 3 (CIN 2/3; n = 148). The methylation pattern for a panel of 15 genes was analysed by quantitative methylation-specific PCR (qMSP) and compared between the groups (≤CIN 1 vs. CIN 2+). Results In the validation set, seven genes presented significantly different methylation profiles according to diagnosis, namely, DAPK1 (p = 0.001), EPB41L3 (p = 0.001), HIC1 (p = 0.028), hsa-miR-124-2 (p = 0.001), LMX1A (p = 0.001), SOX1 (p = 0.001), and TERT (p = 0.001). Six genes showed a significant increase in the frequency of methylation in the presence of hr-HPV, namely, DAPK1 (p = 0.001), EPB41L3 (p = 0.001), hsa-miR-124-2 (p = 0.001), LMX1A (p = 0.001), SOX1 (p = 0.001), and TERT (p = 0.001). The methylation of the hsa-miR-124 gene showed sensitivity and specificity (86.7% and 61.3%, respectively) similar to that of the HPV test (91.3% and 50.0%, respectively). The independent factors associated with the diagnosis of CIN 2+ and the methylation of the hsa-miR-124-2 (OR = 5.1), SOX1 (OR = 2.8), TERT (OR = 2.2), and LMX1A (OR = 2.0) genes were a positive test for hr-HPV (odds ratio [OR] = 5.5). Conclusions Hypermethylation of the hsa-miR-124-2, SOX1, TERT, and LMX1A genes may be a promising biomarker for precursor lesions in cervical cancer regardless of the hr-HPV status. | por |
dc.description.sponsorship | São Paulo Research Foundation (Fundação de Amparo à Pesquisa do Estado de São Paulo–FAPESP) with the cooperation of the Coordination for the Improvement of Higher Education Personnel (Conselho Nacional de Desenvolvimento Científico e Tecnológico–CNPq-PPSUS) (Protocol Nos. 12/51221-4, 14/50014-0, 14/24415-8, and 15/15065-6), the National Institute of Science and Technology (Instituto Nacional de Ciência e Tecnologia–INCT-HPV) (FAPESP Proc. No. 2008/578891 and CNPq Proc. No. 573799/2008-3), and CAPES (PROSUP/CAPES Protocol No. 1571815) | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | restrictedAccess | por |
dc.subject | Adult | por |
dc.subject | Biomarkers, Tumor | por |
dc.subject | Cervical Intraepithelial Neoplasia | por |
dc.subject | Early Detection of Cancer | por |
dc.subject | Female | por |
dc.subject | Humans | por |
dc.subject | LIM-Homeodomain Proteins | por |
dc.subject | MicroRNAs | por |
dc.subject | Middle Aged | por |
dc.subject | Papillomaviridae | por |
dc.subject | Papillomavirus Infections | por |
dc.subject | Promoter Regions, Genetic | por |
dc.subject | SOXB1 Transcription Factors | por |
dc.subject | Sensitivity and Specificity | por |
dc.subject | Telomerase | por |
dc.subject | Transcription Factors | por |
dc.subject | Uterine Cervical Neoplasms | por |
dc.subject | DNA Methylation | por |
dc.subject | Cervical cancer prevention | por |
dc.subject | Cervical cytology | por |
dc.subject | Human papillomavirus | por |
dc.subject | Tumour suppressor genes | por |
dc.title | Methylation of the hsa-miR-124, SOX1, TERT, and LMX1A genes as biomarkers for precursor lesions in cervical cancer | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0090825818309909 | por |
oaire.citationStartPage | 545 | por |
oaire.citationEndPage | 551 | por |
oaire.citationIssue | 3 | por |
oaire.citationVolume | 150 | por |
dc.identifier.eissn | 1095-6859 | - |
dc.identifier.doi | 10.1016/j.ygyno.2018.06.014 | por |
dc.identifier.pmid | 29960712 | por |
dc.subject.fos | Ciências Médicas::Medicina Básica | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Gynecologic Oncology | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
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