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https://hdl.handle.net/1822/62245
Título: | Transient ipsilateral retinal ganglion cell projections to the brain: Extent, targeting, and disappearance |
Autor(es): | Soares, Célia A. Mason, Carol A. |
Palavras-chave: | Animals Axons Brain Cohort Studies Electroporation Functional Laterality Immunohistochemistry Mice, Inbred C57BL Neuroanatomical Tract-Tracing Techniques Retinal Ganglion Cells Visual Pathways dorsocentral retina in utero electroporation optic tract lateral geniculate nucleus superior colliculus |
Data: | Dez-2015 |
Editora: | Wiley |
Revista: | Developmental Neurobiology |
Citação: | Soares, C. A., & Mason, C. A. (2015). Transient ipsilateral retinal ganglion cell projections to the brain: Extent, targeting, and disappearance. Developmental neurobiology, 75(12), 1385-1401. |
Resumo(s): | During development of the mammalian eye, the first retinal ganglion cells (RGCs) that extend to the brain are located in the dorsocentral (DC) retina. These RGCs extend to either ipsilateral or contralateral targets, but the ipsilateral projections do not survive into postnatal periods. The function and means of disappearance of the transient ipsilateral projection are not known. We have followed the course of this transient early ipsilateral cohort of RGCs, paying attention to how far they extend, whether they enter targets and if so, which ones, and the time course of their disappearance. The DC ipsilateral RGC axons were traced using DiI labeling at E13.5 and E15.5 to compare the proportion of ipsi- versus contralateral projections during the first period of growth. In utero electroporation of E12.5 retina with GFP constructs was used to label axons that could be visualized at succeeding time points into postnatal ages. Our results show that the earliest ipsilateral axons grow along the cellular border of the brain, and are segregated from the laterally positioned contralateral axons from the same retinal origin. In agreement with previous reports, although many early RGCs extend ipsilaterally, after E16 their number rapidly declines. Nonetheless, some ipsilateral axons from the DC retina enter the superior colliculus and arborize minimally, but very few enter the dorsal lateral geniculate nucleus and those that do extend only short branches. While the mechanism of selective axonal disappearance remains elusive, these data give further insight into establishment of the visual pathways. |
Tipo: | Artigo |
Descrição: | Author Manuscript |
URI: | https://hdl.handle.net/1822/62245 |
DOI: | 10.1002/dneu.22291 |
ISSN: | 1932-8451 |
e-ISSN: | 1932-846X |
Versão da editora: | https://onlinelibrary.wiley.com/doi/full/10.1002/dneu.22291 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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nihms702356.pdf | 2,32 MB | Adobe PDF | Ver/Abrir |