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https://hdl.handle.net/1822/62340
Título: | Evaluation of tenogenic differentiation potential of selected subpopulations of human adipose‐derived stem cells |
Autor(es): | Gonçalves, A I Berdecka, Dominika Rodrigues, Márcia T. Eren, Aysegul Dede Boer, Jan de Reis, R. L. Gomes, Manuela E. |
Palavras-chave: | Adipose derived stromal/stem cells Subpopulation Tenogenic differentiation Tenomodulin Transforming growth factor beta 3 adipose derived stromal stem cells adipose derived stromal/stem cells, subpopulation, tenogenic differentiation, tenomodulin, transforming growth factor beta 3 |
Data: | Out-2019 |
Editora: | Wiley |
Revista: | Journal of Tissue Engineering and Regenerative Medicine |
Citação: | Gonçalves A. I., Berdecka D., Rodrigues M. T., Eren A. D., Boer J., Reis R. L., Gomes M. E. Evaluation of tenogenic differentiation potential of selected subpopulations of human adipose†derived stem cells, J Tissue Eng Regen Med., pp. 1-14, doi:10.1002/term.2967, 2019 |
Resumo(s): | Identification of a suitable cell source and bioactive agents guiding cell differentiationtowards tenogenic phenotype represents a prerequisite for advancement of cellâ based therapies for tendon repair. Human adiposeâ derived stem cells (hASCs) are apromising, yet intrinsically heterogenous population with diversified differentiationcapacities. In this work, we investigated antigenicallyâ defined subsets of hASCsexpressing markers related to tendon phenotype or associated with pluripotency thatmight be more prone to tenogenic differentiation, when compared to unsortedhASCs. Subpopulations positive for tenomodulin (TNMD+ hASCs) and stage specificearly antigen 4 (SSEAâ 4+ hASCs), as well as unsorted ASCs were cultured up to 21days in basic medium or media supplemented with TGFâ β3 (10 ng/ml), or GDFâ 5(50 ng/ml). Cell response was evaluated by analysis of expression of tendonâ relatedmarkers at gene level and protein level by real time RTâ PCR, western blot, and immu-nocytochemistry. A significant upregulation of scleraxis was observed for both sub-populations and unsorted hASCs in the presence of TGFâ β3. More prominentalterations in gene expression profile in response to TGFâ β3 were observed forTNMD+ hASCs. Subpopulations evidenced an increased collagen III and TNC deposi-tion in basal medium conditions in comparison with unsorted hASCs. In the particularcase of TNMD+ hASCs, GDFâ 5 seems to influence more the deposition of TNC.Within hASCs populations, discrete subsets could be distinguished offering variedsensitivity to specific biochemical stimulation leading to differential expression oftenogenic components suggesting that cell subsets may have distinctive roles in thecomplex biological responses leading to tenogenic commitment to be further exploredin cell based strategies for tendon tissues. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/62340 |
DOI: | 10.1002/term.2967 |
ISSN: | 1932-6254 |
e-ISSN: | 1932-7005 |
Versão da editora: | https://onlinelibrary.wiley.com/doi/pdf/10.1002/term.2967 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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GonçalvesAI et al_JTERM 2019.pdf | 15,1 MB | Adobe PDF | Ver/Abrir |