Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/64317
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Campo DC | Valor | Idioma |
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dc.contributor.author | Ribeiro, Henrique G. | por |
dc.contributor.author | Melo, Luís Daniel Rodrigues | por |
dc.contributor.author | Oliveira, Hugo Alexandre Mendes | por |
dc.contributor.author | Boon, Maarten | por |
dc.contributor.author | Lavigne, Rob | por |
dc.contributor.author | Noben, Jean-Paul | por |
dc.contributor.author | Azeredo, Joana | por |
dc.contributor.author | Oliveira, A. | por |
dc.date.accessioned | 2020-03-09T09:28:47Z | - |
dc.date.available | 2020-03-09T09:28:47Z | - |
dc.date.issued | 2019-12-30 | - |
dc.identifier.citation | Ribeiro, Henrique G.; Melo, Luís D. R.; Oliveira, Hugo; Boon, Maarten; Lavigne, Rob; Noben, Jean-Paul; Azeredo, Joana; Oliveira, A., Characterization of a new podovirus infecting Paenibacillus larvae. Scientific Reports, 9(20355), 2019 | por |
dc.identifier.issn | 2045-2322 | por |
dc.identifier.uri | https://hdl.handle.net/1822/64317 | - |
dc.description.abstract | The Paenibacillus larvae infecting phage API480 (vB_PlaP_API480) is the first reported podovirus for this bacterial species, with an 58nm icosahedral capsid and a 12×8nm short, non-contractile tail. API480 encodes 77 coding sequences (CDSs) on its 45,026bp dsDNA genome, of which 47 were confirmed using mass spectrometry. This phage has got very limited genomic and proteomic similarity to any other known ones registered in public databases, including P. larvae phages. Comparative genomics indicates API480 is a new species as its a singleton with 28 unique proteins. Interestingly, the lysis module is highly conserved among P. larvae phages, containing a predicted endolysin and two putative holins. The well kept overall genomic organisation (from the structural and morphogenetic modules to the host lysis, DNA replication and metabolism related proteins) confirms a common evolutionary ancestor among P. larvae infecting phages. API480 is able to infect 69% of the 61 field strains with an ERIC I genotype, as well as ERIC II strains. Furthermore, this phage is very stable when exposed to high glucose concentrations and to larval gastrointestinal conditions. This highly-specific phage, with its broad lytic activity and stability in hive conditions, might potentially be used in the biocontrol of American Foulbrood (AFB). | por |
dc.description.sponsorship | Te authors would like to aknowledge Dr. Mariano Higes Pascual from Laboratorio de Patología Apícola del Centro de Investigación Apícola y Agroambiental de Marchamalo (CIAPA-IRIAF) for gently providing us P. larvae isolates and the soil sample from which API480 was isolated. Tis study, carried out under the scope of the project APILYSE, PTDC/CVT-EPI/4008/2014 - POCI-01–0145-FEDER-016598, was supported by FCT - Fundação para a Ciência e a Tecnologia, under the scope of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020- Programa Operacional Regional do Norte. HR was supported by FCT through the grant SFRH/BD/128859/2017. MB and RL were supported by KU Leuven through a GOA grant [3E140356]. | por |
dc.language.iso | eng | por |
dc.publisher | Nature Research | por |
dc.relation | PTDC/CVT-EPI/4008/2014 | por |
dc.relation | UID/BIO/04469/2019 | por |
dc.relation | SFRH/BD/128859/2017 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.title | Characterization of a new podovirus infecting Paenibacillus larvae | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.nature.com/srep/index.html | por |
dc.comments | CEB53374 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 9 | por |
dc.date.updated | 2020-03-07T13:36:18Z | - |
dc.identifier.eissn | 2045-2322 | por |
dc.identifier.doi | 10.1038/s41598-019-56699-y | por |
dc.identifier.pmid | 31889094 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Scientific Reports | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53374_1.pdf | 2,12 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons