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https://hdl.handle.net/1822/66347
Título: | Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44 |
Autor(es): | Amorim, Sara Pashkuleva, I. Reis, Celso A. Reis, R. L. Pires, R. A. |
Palavras-chave: | Bioactivity CD44 Hyaluronic acid |
Data: | Mar-2020 |
Editora: | Royal Society of Chemistry |
Revista: | Journal of Materials Chemistry B |
Citação: | Amorim S., Pashkuleva I., Reis C. A., Reis R. L., Pires R. A. Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44, Journal Of Materials Chemistry B, Vol. 17, doi:10.1039/d0tb00407c, 2020 |
Resumo(s): | We report on the development of layer-by-layer (LbL) constructs whose viscoelastic properties and bioactivity can be finely tuned by using polyanions of different size and/or crosslinking. As a polyanion we used hyaluronic acid (HA) â a multi-signaling biomolecule whose bioactivity depends on its molecular weight. We investigated the interplay between the mechanical properties of the LbL systems built using HA of different sizes and the specific HA-mediated biochemical interactions. We characterized the assembled materials and their interactions with CD44, the main HA receptor, by Quartz Crystal Microbalance with Dissipation (QCM-D), Surface Plasmon Resonance (SPR) and Atomic Force Microscopy (AFM). We observed that the presence of CD44 resulted in the disruption of the non-crosslinked multilayers, while crosslinked films remain stable and bind CD44 in a HA molecular weight and charge specific fashion. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/66347 |
DOI: | 10.1039/d0tb00407c |
ISSN: | 2050-750X |
e-ISSN: | 2050-7518 |
Versão da editora: | https://pubs.rsc.org/en/content/articlelanding/2020/tb/d0tb00407c/unauth#!divAbstract |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20275-d0tb00407c.pdf | 2,58 MB | Adobe PDF | Ver/Abrir |