Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/66914
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Mendes, Bárbara Bruna da Silva | por |
dc.contributor.author | Gomez-Florit, Manuel | por |
dc.contributor.author | Araújo, A. C. | por |
dc.contributor.author | Prada, J. | por |
dc.contributor.author | Babo, Pedro Miguel Sousa | por |
dc.contributor.author | Domingues, Rui Miguel Andrade | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Gomes, Manuela E. | por |
dc.date.accessioned | 2020-09-10T16:20:12Z | - |
dc.date.available | 2021-08-01T06:00:32Z | - |
dc.date.issued | 2020-07 | - |
dc.date.submitted | 2020 | - |
dc.identifier.citation | Mendes B. B., Gómez-Florit M., Araújo A. C., Prada J., Babo P. S., Domingues R. M. A., Reis R. L., Gomes M. E. Intrinsically bioactive cryogels based on platelet lysate nanocomposites for hemostasis applications. , Biomacromolecules, doi:10.1021/acs.biomac.0c00787, 2020 | por |
dc.identifier.issn | 1526-4602 | por |
dc.identifier.uri | https://hdl.handle.net/1822/66914 | - |
dc.description.abstract | The currently used hemostatic agents are highly effective in stopping hemorrhages but have a limited role in the modulation of the wound-healing environment. Herein, we propose an intrinsically bioactive hemostatic cryogel based on platelet lysate (PL) and aldehyde-functionalized cellulose nanocrystals (a-CNCs). PL has attracted great attention as an inexpensive milieu of therapeutically relevant proteins; however, its application as a hemostatic agent exhibits serious constraints (e.g., structural integrity and short shelf-life). The incorporation of a-CNCs reinforced the low-strength PL matrix by covalent cross-linking its amine groups that exhibit an elastic interconnected porous network after full cryogelation. Upon blood immersion, the PL-CNC cryogels absorbed higher volumes of blood at a faster rate than commercial hemostatic porcine gelatin sponges. Simultaneously, the cryogels released biomolecules that increased stem cell proliferation, metabolic activity, and migration as well as downregulated the expression of markers of the fibrinolytic process. In an in vivo liver defect model, PL-CNC cryogels showed similar hemostatic performance in comparison with gelatin sponges and normal material-induced tissue response upon subcutaneous implantation. Overall, owing to their structure and bioactive composition, the proposed PL-CNC cryogels provide an alternative off-the-shelf hemostatic and antibacterial biomaterial with the potential to deliver therapeutically relevant proteins in situ. | por |
dc.description.sponsorship | The authors thank Hospital da Prelada (Porto, Portugal) for providing adipose tissue samples and Instituto Portugues do Sangue e Transplantacio-IPST (Portugal) (Porto, Portugal) for providing platelet concentrates. The authors would like to thank Alain Morais and Isabel Pires for their support in the in vivo procedure and histological evaluation, respectively. The authors would like to thank the anonymous reviewers for all useful and helpful comments on our manuscript. This work was supported by the European Research Council grant agreement no. 772817, FCT/MCTES (Fundacio para a Ciencia e a Tecnologia/Ministerio da Ciencia, Tecnologia, e Ensino Superior) and the Fundo Social Europeu atraves do Programa Operacional do Capital Humano (FSE/POCH) in the framework of Ph.D. grant PD/59/2013-PD/BD/113807/2015 (BBM) and CEECIND/01375/2017 (MGF), Norwegian Research Council for project no. 287953. | por |
dc.language.iso | eng | por |
dc.publisher | ACS Publications | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/772817/EU | - |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/PD%2FBD%2F113807%2F2015/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F01375%2F2017%2FCP1458%2FCT0023/PT | - |
dc.rights | openAccess | por |
dc.subject | Cryogels | por |
dc.subject | Hemostatic agent | por |
dc.subject | Platelet lysate | por |
dc.title | Intrinsically bioactive cryogels based on platelet lysate nanocomposites for hemostasis applications. | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://pubs.acs.org/doi/abs/10.1021/acs.biomac.0c00787 | por |
dc.comments | http://3bs.uminho.pt/node/20375 | por |
oaire.citationStartPage | 3678 | por |
oaire.citationEndPage | 3692 | por |
oaire.citationIssue | 9 | por |
oaire.citationVolume | 21 | por |
dc.date.updated | 2020-09-08T15:11:32Z | - |
dc.identifier.doi | 10.1021/acs.biomac.0c00787 | por |
dc.identifier.pmid | 32786530 | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biomacromolecules | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20375-Manuscript_PDF file.pdf | 1,55 MB | Adobe PDF | Ver/Abrir |