Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/70438
Título: | Designing P. aeruginosa synthetic phages with reduced genomes |
Autor(es): | Pires, Diana Priscila Penso Monteiro, Rodrigo Mil-Homens, Dalila Fialho, Arsénio Lu, Timothy K. Azeredo, Joana |
Data: | 25-Jan-2021 |
Editora: | Nature Publishing Group |
Revista: | Scientific Reports |
Citação: | Pires, Diana P.; Monteiro, Rodrigo; Mil-Homens, Dalila; Fialho, Arsénio; Lu, Timothy K.; Azeredo, Joana, Designing P. aeruginosa synthetic phages with reduced genomes. Scientific Reports, 11(2164), 2021 |
Resumo(s): | In the era where antibiotic resistance is considered one of the major worldwide concerns, bacteriophages have emerged as a promising therapeutic approach to deal with this problem. Genetically engineered bacteriophages can enable enhanced anti-bacterial functionalities, but require cloning additional genes into the phage genomes, which might be challenging due to the DNA encapsulation capacity of a phage. To tackle this issue, we designed and assembled for the first time synthetic phages with smaller genomes by knocking out up to 48\% of the genes encoding hypothetical proteins from the genome of the newly isolated Pseudomonas aeruginosa phage vB\_PaeP\\_PE3. The antibacterial efficacy of the wild-type and the synthetic phages was assessed in vitro as well as in vivo using a Galleria mellonella infection model. Overall, both in vitro and in vivo studies revealed that the knock-outs made in phage genome do not impair the antibacterial properties of the synthetic phages, indicating that this could be a good strategy to clear space from phage genomes in order to enable the introduction of other genes of interest that can potentiate the future treatment of P. aeruginosa infections. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/70438 |
DOI: | 10.1038/s41598-021-81580-2 |
ISSN: | 2045-2322 |
Versão da editora: | http://www.nature.com/srep/index.html |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54146_1.pdf | 2,1 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons