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https://hdl.handle.net/1822/70587
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Campo DC | Valor | Idioma |
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dc.contributor.author | Gonçalves, Bruna | por |
dc.contributor.author | Azevedo, Nuno | por |
dc.contributor.author | Osório, Hugo | por |
dc.contributor.author | Henriques, Mariana | por |
dc.contributor.author | Silva, Sónia Carina | por |
dc.date.accessioned | 2021-03-09T10:20:55Z | - |
dc.date.available | 2021-03-09T10:20:55Z | - |
dc.date.issued | 2021-02-26 | - |
dc.identifier.citation | Gonçalves, Bruna; Azevedo, N.; Osório, Hugo; Henriques, Mariana; Silva, Sónia Carina, Revealing Candida glabrata biofilm matrix proteome: global characterization and pH response.. Biochemical Journal, 478(4), 961-967, 2021 | por |
dc.identifier.issn | 02646021 | por |
dc.identifier.uri | https://hdl.handle.net/1822/70587 | - |
dc.description.abstract | Candida glabrata is a clinically relevant human pathogen with the ability to form high recalcitrant biofilms that contribute to the establishment and persistence of infection. A defining trait of biofilms is the auto-produced matrix, which is suggested to have structural, virulent and protective roles. Thus, elucidation of matrix components, their function and modulation by the host environment is crucial to disclose their role in C. glabrata pathogenesis. As a major step toward this end, this study aimed to reveal, for the first time, the matrix proteome of C. glabrata biofilms, to characterize it with bioinformatic tools and to study its modulation by the environmental pH (acidic and neutral). The results showed the presence of several pH-specific matrix proteins (51 acidic- and 206 neutral-specific) and also proteins commonly found at both pH conditions (236). Of note, several proteins related to mannan and -glucan metabolism, which have a potential role in the delivery/organization of carbohydrates in the matrix, were found in both pH conditions but in much higher quantity under the neutral environment. Additionally, several virulence-related proteins, including epithelial adhesins, yapsins and moonlighting enzymes, were found among matrix proteins. Importantly, several proteins seem to have a non-canonical secretion pathway and Pdr1 was found to be a potential regulator of matrix proteome. Overall, this study indicates a relevant impact of environmental cues in the matrix proteome and provides a unique resource for further functional investigation of matrix proteins, contributing to the identification of potential targets for the development of new therapies against C. glabrata biofilms. | por |
dc.description.sponsorship | This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope ofthe strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004)funded by the European Regional Development Fund under the scope of Norte2020—Programa OperacionalRegional do Norte. This work was also supported by a FCT PhD grant [FRH/BD/111645/2015]. The authors alsoacknowledge the project funding by the‘02/SAICT/2017—Projetos de Investigação Científica eDesenvolvimento Tecnológico (IC&DT)—POCI-01-0145-FEDER-028893’. Additionally, this work wasfinancedby the Portuguese Mass Spectrometry Network, integrated in the National Roadmap of Research Infrastructuresof Strategic Relevance [ROTEIRO/0028/2013; LISBOA-01-0145-FEDER-022125] | por |
dc.language.iso | eng | por |
dc.publisher | Portland Press | por |
dc.relation | UID/BIO/04469/2019 | por |
dc.relation | SFRH/BD/111645/2015 | por |
dc.rights | openAccess | por |
dc.subject | adhesins | por |
dc.subject | ß-glucans | por |
dc.subject | biofilm secretome | por |
dc.subject | candidiasis | por |
dc.subject | environmental pH | por |
dc.subject | yapsins | por |
dc.title | Revealing Candida glabrata biofilm matrix proteome: global characterization and pH response. | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://portlandpress.com/biochemj/article-abstract/478/4/961/227820/Revealing-Candida-glabrata-biofilm-matrix-proteome?redirectedFrom=fulltext | por |
dc.comments | CEB54269 | por |
oaire.citationStartPage | 961 | por |
oaire.citationEndPage | 974 | por |
oaire.citationIssue | 4 | por |
oaire.citationConferencePlace | United Kingdom | - |
oaire.citationVolume | 478 | por |
dc.date.updated | 2021-03-09T10:16:36Z | - |
dc.identifier.doi | 10.1042/BCJ20200844 | por |
dc.identifier.pmid | 33555340 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biochemical Journal | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54269_1.pdf | 1,48 MB | Adobe PDF | Ver/Abrir |