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https://hdl.handle.net/1822/71039
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Campo DC | Valor | Idioma |
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dc.contributor.author | Magalhães, Carla Isabel Pereira | por |
dc.contributor.author | Ribeiro, Joaquim Alfredo França Martins | por |
dc.contributor.author | Guedes, Ana P. | por |
dc.contributor.author | Arantes, Ana Luísa | por |
dc.contributor.author | Sousa, Diana Zita Machado | por |
dc.contributor.author | Stams, Alfons Johannes Maria | por |
dc.contributor.author | Alves, M. M. | por |
dc.contributor.author | Cavaleiro, Ana Júlia | por |
dc.date.accessioned | 2021-03-26T17:58:32Z | - |
dc.date.available | 2021-03-26T17:58:32Z | - |
dc.date.issued | 2020-07 | - |
dc.identifier.citation | Magalhães, C. P.; Ribeiro, J.; Guedes, Ana P.; Arantes, Ana Luísa; Sousa, Diana Z.; Stams, A. J. M.; Alves, M. Madalena; Cavaleiro, Ana Júlia, Co-cultivation of Thermoanaerobacter strains with a methanogenic partner enhances glycerol conversion. Microbial Biotechnology, 13(4), 962-973, 2020 | por |
dc.identifier.issn | 1751-7915 | por |
dc.identifier.uri | https://hdl.handle.net/1822/71039 | - |
dc.description.abstract | Glycerolrich waste streams produced by the biodiesel, bioethanol and oleochemical industries can be treated and valorized by anaerobic microbial communities to produce methane. As current knowledge of the microorganisms involved in thermophilic glycerol conversion to methane is scarce, thermophilic glyceroldegrading methanogenic communities were enriched. A coculture of Thermoanaerobacter and Methanothermobacter species was obtained, pointing to a nonobligately syntrophic glycerol degradation. This hypothesis was further studied by incubating Thermoanaerobacter brockii subsp. finnii and T. wiegelii with glycerol (10 mM) in pure culture and with different hydrogenotrophic methanogens. The presence of the methanogen accelerated glycerol fermentation by the two Thermoanaerobacter strains up to 3.3 mM day1, corresponding to 12 times higher volumetric glycerol depletion rates in the methanogenic cocultures than in the pure bacterial cultures. The catabolic pathways of glycerol conversion were identified by genome analysis of the two Thermoanaerobacter strains. NADH and reduced ferredoxin formed in the pathway are linked to proton reduction, which becomes thermodynamically favourable when the hydrogen partial pressure is kept low by the hydrogenotrophic methanogenic partner. | por |
dc.description.sponsorship | The authors thank Ruben Gonçalves for preparing the thermophilic biomass and Andreia Salvador for the sup port with the microbial communities’ analysis. This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit, Project SAICTPAC/0040/2015 (POCI-01-0145-FEDER-016403) and BioTecNorte operation (NORTE-01-0145-FEDER 000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Opera cional Regional do Norte. The authors also acknowledge the financial support of FCT and European Social Fund through the grants attributed to C.P. Magalhaes (SFRH/BD/132845/2017) and A.L. Arantes (PD/BD/128030/2016). | por |
dc.language.iso | eng | por |
dc.publisher | Wiley-Blackwell | por |
dc.relation | UID/BIO/04469/2019 | por |
dc.relation | SAICTPAC/0040/2015 | por |
dc.relation | POCI-01-0145-FEDER-016403 | por |
dc.relation | SFRH/BD/132845/2017 | por |
dc.relation | PD/BD/128030/2016 | por |
dc.rights | openAccess | por |
dc.title | Co-cultivation of Thermoanaerobacter strains with a methanogenic partner enhances glycerol conversion | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/journal/17517915 | por |
dc.comments | CEB53589 | por |
oaire.citationStartPage | 962 | por |
oaire.citationEndPage | 973 | por |
oaire.citationIssue | 4 | por |
oaire.citationVolume | 13 | por |
dc.date.updated | 2021-02-15T17:28:11Z | - |
dc.identifier.doi | 10.1111/1751-7915.13506 | por |
dc.identifier.pmid | 32154666 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Microbial Biotechnology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53589_1.pdf | 754,39 kB | Adobe PDF | Ver/Abrir |