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https://hdl.handle.net/1822/71279
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Campo DC | Valor | Idioma |
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dc.contributor.author | Vilaça, Natália | por |
dc.contributor.author | Bertão, Ana Raquel | por |
dc.contributor.author | Prasetyanto, Eko Adi | por |
dc.contributor.author | Granja, Sara | por |
dc.contributor.author | Costa, Marta | por |
dc.contributor.author | Fernandes, Rui | por |
dc.contributor.author | Figueiredo, Francisco | por |
dc.contributor.author | Fonseca, António | por |
dc.contributor.author | Cola, Luisa De | por |
dc.contributor.author | Baltazar, Fátima | por |
dc.contributor.author | Neves, Isabel C. | por |
dc.date.accessioned | 2021-04-05T20:28:38Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Vilaça, Natália; Bertão, Ana Raquel; Prasetyanto, Eko Adi; Granja, Sara; Costa, Marta; Fernandes, Rui; Figueiredo, Francisco; Fonseca, António; Cola, Luisa De; Baltazar, Fátima; Neves, Isabel C., Surface functionalization of zeolite-based drug delivery systems enhances their antitumoral activity in vivo. Materials Science and Engineering C-Materials for Biological Applications, 120(111721), 2021. | por |
dc.identifier.issn | 0928-4931 | por |
dc.identifier.uri | https://hdl.handle.net/1822/71279 | - |
dc.description | Supplementary data to this article can be found online at https://doi. org/10.1016/j.msec.2020.111721. | por |
dc.description.abstract | Zeolites have attractive features making them suitable carriers for drug delivery systems (DDS). As such, we loaded the anticancer drug 5-fluorouracil (5-FU), into two different zeolite structures, faujasite (NaY) and Linde Type L (LTL), to obtain different DDS. The prepared DDS were tested in vitro using breast cancer, colorectal carcinoma, and melanoma cell lines and in vivo using the chick embryo chorioallantoic membrane model (CAM). Both assays showed the best results for the Hs578T breast cancer cells, with a higher potentiation for 5-FU encapsulated in the zeolite LTL. To unveil the endocytic mechanisms involved in the internalization of the zeolite nanoparticles, endocytosis was inhibited pharmacologically in breast cancer and epithelial mammary human cells. The results suggest that a caveolin-mediated process was responsible for the internalized zeolite nanoparticles. Aiming to boost the DDS efficacy, the disc-shaped zeolite LTL outer surface was functionalized using amino (NH2) or carboxylic acid (COOH) groups and coated with poly-L-lysine (PLL). Positively functionalized surface LTL nanoparticles revealed to be non-toxic to human cells and, importantly, their internalization was faster and led to a higher tumor reduction in vivo. Overall, our results provide further insights into the mechanisms of interaction between zeolite-based DDS and cancer cells, and pave the way for future studies aiming to improve DDS anticancer activity. | por |
dc.description.sponsorship | NV and ARB thank FCT (Foundation for Science and Technology) for their Ph.D. grants (SFRH/BD/97797/2013 and SFRH/BD/141058/2018, respectively). This work has been funded by ICVS Scientific Mi croscopy Platform, member of the national infrastructure PPBI - Portu guese Platform of Bioimaging (PPBI-POCI-01-0145-FEDER-022122); by National funds, through the Foundation for Science and Technology (FCT) - project UIDB/50026/2020 and UIDP/50026/2020; and by the projects NORTE-01-0145-FEDER-000013 and NORTE-01-0145-FEDER 000023, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work was also supported by FCT, under the scope of the projects: PTDC/ AAGTEC/5269/2014 and Centre of Chemistry (UID/QUI/00686/2013 and UID/QUI/0686/2016). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier | por |
dc.relation | SFRH/BD/97797/2013 | por |
dc.relation | SFRH/BD/141058/2018 | por |
dc.relation | UIDB/50026/2020 | por |
dc.relation | UIDP/50026/2020 | por |
dc.relation | UID/QUI/00686/2013 | por |
dc.relation | UID/QUI/0686/2016 | por |
dc.rights | restrictedAccess | por |
dc.subject | Zeolite nanoparticles | por |
dc.subject | drug delivery systems (DDS) | por |
dc.subject | Surface functionalization | por |
dc.subject | cancer models | por |
dc.subject | Chick Chorioallantoic Membrane (CAM) assay | por |
dc.title | Surface functionalization of zeolite-based drug delivery systems enhances their antitumoral activity in vivo | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/materials-science-and-engineering-c/ | por |
dc.comments | CEB54000 | por |
oaire.citationIssue | 111721 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationVolume | 120 | por |
dc.date.updated | 2021-02-15T17:27:15Z | - |
dc.identifier.doi | 10.1016/j.msec.2020.111721 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 33545872 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Materials Science and Engineering: C Materials for Biological Applications | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series ICVS - Artigos em revistas internacionais / Papers in international journals CDQuim - Artigos (Papers) |
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