Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/72768
Título: | Bioactivity of biosilica obtained from North Atlantic deep-sea sponges |
Autor(es): | Dudik, Olesia Amorim, Sara Xavier, Joana Rute Calça Rapp, Hans Tore Silva, Tiago H. Pires, R. A. Reis, R. L. |
Palavras-chave: | Bioactivity Biosilica Bioceramics Tissue engineering Deep-sea sponges |
Data: | 17-Mai-2021 |
Editora: | Frontiers Media S.A. |
Revista: | Frontiers in Marine Science |
Citação: | Dudik O., Amorim S., Xavier J. R., Rapp H. T., Silva T. H., Pires R. A., Reis R. L. Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges, Frontiers in Marine Science, Vol. 8, pp. 637810, doi:10.3389/fmars.2021.637810, 2021 |
Resumo(s): | Demosponges are a well-known source of a plethora of bioactive compounds. In particular, they are able to form a skeleton by direct deposition of silica in a process catalysed by silicatein. Herein, we isolated biosilicas from five different Atlantic deep-sea sponges Geodia atlantica (GA), Geodia barretti (GB), Stelletta normani (SN), Axinella infundibuliformis (AI) and Phakellia ventilabrum (PV) to explore the bioactivity and osteogenic capacity of its silica-based materials. We chemically characterized the isolated biosilicas and evaluated them for their bioactivity to deposit Ca and P on their surface (by immersion in simulated body fluid, SBF). GB-, SN-, AI- and PV-based biosilicas did not generate a stable calcium phosphate (CaP) layer over time in the presence of SBF, however, the GA-derived one was able to form a CaP surface layer (at a Ca/P ratio of ~1.7, similar to the one observed for hydroxyapatite), that was stable during the 28 days of testing. In addition, no cytotoxicity towards L929 and SaOs2 cells was observed for the GA-based biosilica up to a concentration of 10 mg/mL. Overall, the GA-based biosilica presents the characteristics to be used in the development of biomaterials for bone tissue engineering (BTE). |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/72768 |
DOI: | 10.3389/fmars.2021.637810 |
ISSN: | 2296-7745 |
Versão da editora: | https://www.frontiersin.org/articles/10.3389/fmars.2021.637810/full |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
20536-dudik-et-al-bioactivity-biosilica-frontiers-2021.pdf | 5,07 MB | Adobe PDF | Ver/Abrir |