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https://hdl.handle.net/1822/72855
Título: | Current status of DNA barcoding of aquatic hyphomycetes |
Autor(es): | Duarte, Sofia Alexandra Ferreira Baerlocher, Felix Cássio, Fernanda Pascoal, Cláudia |
Palavras-chave: | Freshwaters Anamorphic fungi Taxonomy Molecular identification |
Data: | 2014 |
Editora: | Verlag Ferdinand Berger Sohne Gesellschaft Mbh |
Revista: | Sydowia |
Citação: | Duarte, S., Baerlocher, F., Cassio, F., & Pascoal, C. (2014). Current status of DNA barcoding of aquatic hyphomycetes. Sydow, 66(2), 191-202 |
Resumo(s): | DNA barcoding is a rapid diagnostic approach in which short DNA sequences (ca. 600 bp) - the DNA barcodes - are used for species identification. Ideally, barcodes are sufficiently variable to distinguish closely related species and are flanked by conserved regions to allow the use of grdup-specffic primers. Fungi play pivotal roles in ecosystems and the adoption of culture-independent methods based on nucleic acids extracted directly from environmental samples has become increasingly important to examine their diversity. Aquatic hyphomycetes are a polyphyletic group of fungi that play a key role in organic matter processing in freshwaters. The internal transcribed spacer (ITS) region from rDNA was designated as the most suitable barcode for fungal identification. In the current study, we accessed all ITS sequences belonging to aquatic hyphomycete species present in GenBank in the International Nucleotide Sequence Database Collaboration (INSDC) (http://www.ncbi.nlm.nih.gov/genbank/). Today, ca. 26 % of all described species have been connected to an ITS barcode. The growth of DNA datasets allows more accurate assessments of biodiversity, and provides greater insights into fungal ecology. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/72855 |
DOI: | 10.12905/0380.sydowia66(2)2014-0191 |
ISSN: | 0082-0598 |
Versão da editora: | https://www.cabdirect.org/cabdirect/abstract/20198653522 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CBMA - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Duarte et al 2014_Sydowia.pdf Acesso restrito! | 6,3 MB | Adobe PDF | Ver/Abrir |