Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/72909
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Oliveira, Isabel S. | por |
dc.contributor.author | Machado, Rui L. | por |
dc.contributor.author | Araujo, Maria J. | por |
dc.contributor.author | Gomes, Andreia C | por |
dc.contributor.author | Marques, Eduardo F. | por |
dc.date.accessioned | 2021-05-27T13:59:43Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Oliveira, I. S., Machado, R. L., Araújo, M. J., Gomes, A. C., & Marques, E. F. (2021). Stimuli‐Sensitive Self‐Assembled Tubules Based on Lysine‐Derived Surfactants for Delivery of Antimicrobial Proteins. Chemistry–A European Journal, 27(2), 692-704 | por |
dc.identifier.issn | 0947-6539 | - |
dc.identifier.uri | https://hdl.handle.net/1822/72909 | - |
dc.description | Accepted Manuscript | por |
dc.description.abstract | Drug delivery vectors based on amphiphiles have important features such as versatile physicochemical properties and stimuli-responsiveness. Amino acid-based surfactants are especially promising amphiphiles due to their enhanced biocompatibility compared to conventional surfactants. They can self-organize into micelles, vesicles and complex hierarchical structures, such as fibers, twisted and coiled ribbons, and tubules. In this work, we investigated the self-assembly and drug loading properties of a family of novel anionic double-tailed lysine-derived surfactants, with variable degree of tail length mismatch, designated as mLys10 and 10Lysn, where m and n are the number of carbon atoms in the tails. These surfactants form tubular aggregates with assorted morphologies in water that undergo gelation due to dense entanglement, as evidenced by light and electron microscopy. Lysozyme (LZM), an enzyme with antimicrobial properties, was selected as model protein for loading. After the characterization of the interfacial properties and phase behavior of the amphiphiles, the LZM-loading ability of the tubules was investigated, under varying experimental conditions, to assess the efficiency of the aggregates as pH- and temperature-sensitive nanocarriers. Further, the toxicological profile of the surfactants per se and surfactant/LZM hydrogels was obtained, using human skin fibroblasts (BJ-5ta cell line). Overall, the results show that the tubule-based hydrogels exhibit very interesting properties for the transport and controlled release of molecules of therapeutic interest. | por |
dc.description.sponsorship | The authors acknowledge FundacAo para a Ciencia e a Tecnologia (FCT) for financial support through projects UIDB/00081/2020 and UIDB/50006/2020. This work was also funded by the ERDF through the COMPETE2020-POCI with the strategic program UID/BIA/04050/2019 (POCI-01-0145-FEDER-007569). We thank R.Fernandes for help with the self-assembly studies, and Prof. O.Regev for help with the cryo-TEM experiments and discussions. I. S. Oliveira also acknowledges financial support from FCT through the Ph.D. grant SFRH/BD/108629/2015. | por |
dc.language.iso | eng | por |
dc.publisher | Wiley-VCH Verlag | por |
dc.rights | closedAccess | por |
dc.subject | Amino acid-based surfactants | por |
dc.subject | Cytotoxicity | por |
dc.subject | Drug delivery | por |
dc.subject | Self-assembly | por |
dc.subject | Stimuli-responsive hydrogel | por |
dc.title | Stimuli-Sensitive self-assembled tubules based on lysine-derived surfactants for delivery of antimicrobial proteins | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/chem.202003320 | por |
oaire.citationStartPage | 692 | por |
oaire.citationEndPage | 704 | por |
oaire.citationIssue | 2 | por |
oaire.citationVolume | 27 | por |
dc.date.updated | 2021-05-24T23:00:26Z | - |
dc.identifier.doi | 10.1002/chem.202003320 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 32830362 | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
dc.subject.fos | Ciências Naturais::Ciências Biológicas | por |
dc.subject.wos | Science & Technology | - |
sdum.export.identifier | 10863 | - |
sdum.journal | Chemistry - A European Journal | por |
Aparece nas coleções: | CBMA - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
chem.202003320.pdf Acesso restrito! | 3,19 MB | Adobe PDF | Ver/Abrir |