Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/72972
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Campo DC | Valor | Idioma |
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dc.contributor.author | Pacheco, Andreia Sofia Alves Pinto | por |
dc.contributor.author | Donzella, Lorena | por |
dc.contributor.author | Hernandez-Lopez, Maria Jose | por |
dc.contributor.author | Almeida, Maria Judite | por |
dc.contributor.author | Prieto, José António | por |
dc.contributor.author | Randez-Gil, Francisca | por |
dc.contributor.author | Morrissey, John P. | por |
dc.contributor.author | Sousa, Maria João | por |
dc.date.accessioned | 2021-05-28T08:50:11Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Pacheco, A., Donzella, L., Hernandez-Lopez, M. J., Almeida, M. J., Prieto, J. A., et al.(2020). Hexose transport in Torulaspora delbrueckii: identification of Igt1, a new dual-affinity transporter. FEMS yeast research, 20(1), foaa004 | por |
dc.identifier.issn | 1567-1356 | - |
dc.identifier.uri | https://hdl.handle.net/1822/72972 | - |
dc.description.abstract | Torulaspora delbrueckii is a yeast species receiving increasing attention from the biotechnology industry, with particular relevance in the wine, beer and baking sectors. However, little is known about its sugar transporters and sugar transport capacity, frequently a rate-limiting step of sugar metabolism and efficient fermentation. Actually, only one glucose transporter, Lgt1, has been characterized so far. Here we report the identification and characterization of a second glucose transporter gene, IGT1, located in a cluster, upstream of LGT1 and downstream of two other putative hexose transporters. Functional characterization of IGT1 in a Saccharomyces cerevisiae hxt-null strain revealed that it encodes a transporter able to mediate uptake of glucose, fructose and mannose and established that its affinity, as measured by Km, could be modulated by glucose concentration in the medium. In fact, IGT1-transformed S. cerevisiae hxt-null cells, grown in 0.1% glucose displayed biphasic glucose uptake kinetics with an intermediate- (Km = 6.5 ± 2.0 mM) and a high-affinity (Km = 0.10 ± 0.01 mM) component, whereas cells grown in 2% glucose displayed monophasic kinetics with an intermediate-affinity (Km of 11.5 ± 1.5 mM). This work contributes to a better characterization of glucose transport in T. delbrueckii, with relevant implications for its exploitation in the food industry. | por |
dc.description.sponsorship | AP was supported by PhD fellowship [BD/13282/2003] from Fundação para a Ciência e para a Tecnologia (FCT), Portugal. LD is supported by the YEASTDOC Marie Curie ITN project which received funding from the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie grant agreement no 764927. This work was supported by the strategic programme UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569) and project PTDC/BIA-MIC/32059/2017 funded by national funds through the FCT,I.P. and by the ERDF through the COMPETE2020 – Programa Operacional Competitividade e Internacionalização (POCI) and Sistema de Apoio à Investigação Científica e Tecnológica (SAICT). | por |
dc.language.iso | eng | por |
dc.publisher | Oxford University Press | por |
dc.rights | restrictedAccess | por |
dc.subject | Fermentation | por |
dc.subject | Fructose | por |
dc.subject | Glucose | por |
dc.subject | Kinetics | por |
dc.subject | Mannose | por |
dc.subject | Monosaccharide Transport Proteins | por |
dc.subject | Saccharomyces cerevisiae | por |
dc.subject | Torulaspora | por |
dc.subject | Carbohydrate Metabolism | por |
dc.subject | Torulaspora delbrueckii | por |
dc.subject | Glucose transport | por |
dc.subject | hexose transporter | por |
dc.subject | transport kinetics | por |
dc.subject | dual affinity | por |
dc.subject | glucose transporter gene cluster | por |
dc.title | Hexose transport in Torulaspora delbrueckii: identification of Igt1, a new dual-affinity transporter | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://academic.oup.com/femsyr/article-abstract/20/1/foaa004/5715911 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 20 | por |
dc.identifier.doi | 10.1093/femsyr/foaa004 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 31981362 | por |
dc.subject.fos | Ciências Naturais::Ciências Biológicas | por |
dc.subject.fos | Engenharia e Tecnologia::Biotecnologia Industrial | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | FEMS Yeast Research | por |
oaire.version | AM | por |
Aparece nas coleções: | CBMA - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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CHARACTERIZATION OF Igt1 main document accepted manuscript.pdf Acesso restrito! | Accepted Version | 2 MB | Adobe PDF | Ver/Abrir |