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dc.contributor.authorSousa, Lúcia Filipa Guimarães Vieirapor
dc.contributor.authorCastro, Joana Isabel Reispor
dc.contributor.authorFrança, Ângela Maria Oliveira Sousapor
dc.contributor.authorAlmeida, Carinapor
dc.contributor.authorMuzny, C. A.por
dc.contributor.authorCerca, Nunopor
dc.identifier.citationSousa, Lúcia; Castro, Joana; França, Angela; Almeida, Carina; Muzny, C. A.; Cerca, Nuno, A new PNA-FISH probe targeting Fannyhessea vaginae. Frontiers in Cellular and Infection Microbiology, 11(779376), 2021por
dc.descriptionThe Supplementary Material for this article can be found online at:
dc.description.abstractBacterial vaginosis (BV) is the most common vaginal infection in women of reproductive age and has been associated with serious health complications, mainly in pregnant women. It is characterized by a decrease in the number of Lactobacillus species in the healthy vaginal microbiota and an overgrowth of strict and facultative anaerobic bacteria that develop a polymicrobial biofilm. Despite over 60 years of research investigating BV, its etiology is not fully understood. Gardnerella spp. is a crucial microorganism that contributes to the formation of the biofilm and the development of BV, but the role of other BV-associated bacteria is not clear. Nevertheless, Fannyhessea vaginae (previously known as Atopobium vaginae) is a highly specific species for BV, and co-colonization with Gardnerella is thought to be a very specific diagnostic marker. The diagnosis of BV still presents some limitations, since currently used methods often fail to accurately detect BV. This work aims to develop a novel peptide nucleic acid (PNA) probe targeting F. vaginae. This probe was further validated in a multiplex assay, which included a Gardnerella-specific PNA probe, as a possible method for diagnosis of BV, and was compared with quantification by qPCR. The new PNA probe showed excellent sensitivity and specificity and could discriminate F. vaginae-Gardnerella biofilms, confirming the potential to be used for the detection of BV-associated pathogens.por
dc.description.sponsorshipThis work was funded by the National Institute of Allergy and Infectious Diseases (R01AI146065-01A1). It was also partially funded by the Portuguese Foundation for Science and Technology (FCT), under the scope of the strategic funding of unit (UIDB/04469/2020).por
dc.publisherFrontiers Media S. A.por
dc.subjectFannyhessea vaginaepor
dc.subjectGardnerella vaginalispor
dc.subjectbacterial vaginosispor
dc.subjectfluorescence in situ hybridization (FISH)por
dc.subjectpeptide nucleic acid (PNA)por
dc.titleA new PNA-FISH probe targeting Fannyhessea vaginaepor
dc.subject.fosCiências Naturais::Ciências Biológicaspor
sdum.journalFrontiers in Cellular and Infection Microbiologypor
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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