Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/75266
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Hurle, K. | por |
dc.contributor.author | Maia, F. Raquel | por |
dc.contributor.author | Ribeiro, Viviana Pinto | por |
dc.contributor.author | Pina, Sandra Cristina Almeida | por |
dc.contributor.author | Oliveira, J. M. | por |
dc.contributor.author | Goetz-Neunhoeffer, F. | por |
dc.contributor.author | Reis, R. L. | por |
dc.date.accessioned | 2022-01-06T16:48:11Z | - |
dc.date.available | 2022-01-06T16:48:11Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Hurle K., Maia F. R., Ribeiro V. P., Pina S., Oliveira J. M., Goetz-Neunhoeffer F., Reis R. L. Osteogenic lithium-doped brushite cements for bone regeneration, Bioactive Materials, doi:10.1016/j.bioactmat.2021.12.025, 2021 | por |
dc.identifier.issn | 2452-199X | por |
dc.identifier.uri | https://hdl.handle.net/1822/75266 | - |
dc.description | Available online 31 December 2021 | por |
dc.description.abstract | This study investigated the osteogenic performance of new brushite cements obtained from Li+-doped beta-tricalcium phosphate as a promising strategy for bone regeneration. Lithium (Li+) is a promising trace element to encourage the migration and proliferation of adipose-derived stem cells (hASCs) and the osteogenic differentiation-related gene expression, essential for osteogenesis. In-situ X-ray diffraction (XRD) and in-situ H-1 nuclear magnetic resonance (H-1 NMR) measurements proved the precipitation of brushite, as main phase, and monetite, indicating that Li+ favored the formation of monetite under certain conditions. Li+ was detected in the remaining pore solution in significant amounts after the completion of hydration. Isothermal calorimetry results showed an accelerating effect of Li+, especially for low concentration of the setting retarder (phytic acid). A decrease of initial and final setting times with increasing amount of Li+ was detected and setting times could be well adjusted by varying the setting retarder concentration. The cements presented compressive mechanical strength within the ranges reported for cancellous bone. In vitro assays using hASCs showed normal metabolic and proliferative levels. The immunodetection and gene expression profile of osteogenic-related markers highlight the incorporation of Li+ for increasing the in vivo bone density. The osteogenic potential of Li-doped brushite cements may be recommended for further research on bone defect repair strategies. | eng |
dc.description.sponsorship | This study was funded by the Portuguese Foundation for Science and Technology (FCT) and the German Academic Exchange Service (Deutscher Akademischer Austauschdienst, DAAD) for the transnational cooperation FCT/DAAD 2018-2019. FRM acknowledges her contract under the Transitional Rule DL 57/2016 (CTTI-57/18-I3BS(5)) attributed by the FCT. VPR acknowledges the Junior Researcher contracts (POCI-01-0145-FEDER-031367; POCI-01-0145-FEDER-029139) under the projects Fun4TE project (PTDC/EMD-EMD/31367/2017) and BLiver (PTDC/EMD-EMD/29139/2017) attributed by the FCT. The authors also thank the funds provided under the distinctions attributed to JMO (IF/01285/2015) and SP (CEECIND/03673/2017). Furthermore, funding by the German Research Foundation (Deutsche Forschungsgemeinschaft, DFG), Grant Nr. HU 2498/1-1; GB 1/22-1, is acknowledged. | por |
dc.language.iso | eng | por |
dc.publisher | KeAi Communications | por |
dc.relation | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FEMD-EMD%2F31367%2F2017/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FEMD-EMD%2F29139%2F2017/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F03673%2F2017%2FCP1458%2FCT0040/PT | por |
dc.rights | openAccess | por |
dc.subject | Alkaline phosphatase activity | por |
dc.subject | Bone regeneration | por |
dc.subject | Brushite cements | por |
dc.subject | Collagen expression | por |
dc.subject | Lithium doping | por |
dc.subject | Osteogenic expression | por |
dc.subject | β-tricalcium phosphate | por |
dc.subject | beta-tricalcium phosphate | por |
dc.title | Osteogenic lithium-doped brushite cements for bone regeneration | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S2452199X21006009 | por |
dc.comments | http://3bs.uminho.pt/node/20677 | por |
oaire.citationStartPage | 403 | por |
oaire.citationEndPage | 417 | por |
oaire.citationVolume | 16 | por |
dc.date.updated | 2022-01-03T12:17:09Z | - |
dc.identifier.doi | 10.1016/j.bioactmat.2021.12.025 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Bioactive Materials | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20677-final-paper.pdf | 12,04 MB | Adobe PDF | Ver/Abrir |