Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/7832
Título: | Differentiation of human pre-adipocytes by recombinant adiponectin |
Autor(es): | Avides, Maria do Carmo Domingues, Lucília Vicente, A. A. Teixeira, J. A. |
Palavras-chave: | Active adiponectin Baculovirus Adipocytes Differentiation Hormone |
Data: | Mai-2008 |
Editora: | Elsevier 1 |
Revista: | Protein Expression and Purification |
Citação: | "Protein Expression and Purification". ISSN 1046-5928. 59:1 (May 2008) 122-126. |
Resumo(s): | Obesity has become a global health problem and it is linked to a higher risk of diseases and metabolic disorders such as diabetes, cardiovascular disease, and cancer. The adipose tissue plays an important role in monitoring and controlling whole-body metabolism by secreting a variety of bioactive molecules such as adiponectin. Deficiencies of this hormone can cause type II diabetes and cardiovascular disease both in mice and in humans. Therefore, adiponectin is an attractive molecule to use in human therapy, particularly in a recombinant form. The source of recombinant adiponectin could be the expression of full-length adiponectin either in Escherichia coli, or in baculovirus. In this work we express and purify human adiponectin in both systems. The adiponectin produced by baculovirus was found to be 10 times more active as far as oligomerization and human pre-adipocyte differentiation are concerned, when compared with adiponectin produced by E. coli. We presume that adiponectin expressed in baculovirus has post-translation modifications not made by bacteria which may be responsible for these differences in activity. This renders adiponectin produced by baculovirus a better candidate for the treatment of type II diabetes and cardiovascular disease. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/7832 |
DOI: | 10.1016/j.pep.2008.01.012 |
ISSN: | 1046-5928 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Avides_PEP[2].pdf | 538,81 kB | Adobe PDF | Ver/Abrir |