Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/78411
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Melro, Liliana Sofia Silva Ferreira Pinto | por |
dc.contributor.author | Tavares, Tânia Daniela Eugénio | por |
dc.contributor.author | Padrão, Jorge | por |
dc.contributor.author | Dourado, Fernando | por |
dc.contributor.author | Gama, F. M. | por |
dc.contributor.author | Silva, Carla Manuela Costa | por |
dc.contributor.author | Antunes, Joana Carolina Simões | por |
dc.contributor.author | Felgueiras, Helena Prado | por |
dc.contributor.author | Zille, Andrea | por |
dc.date.accessioned | 2022-06-15T11:28:57Z | - |
dc.date.available | 2022-06-15T11:28:57Z | - |
dc.date.issued | 2022 | - |
dc.identifier.uri | https://hdl.handle.net/1822/78411 | - |
dc.description.abstract | Burn wounds can lead to numerous severe complications including bacterial infections causing patient morbidity and mortality, mostly in low- and middle-income countries. The considerable increase of the microbial resistance against traditional antibiotics is leading towards alternative strategies to treat bacterial infections. Nisin Z is an antimicrobial peptide which exhibits a significant antibacterial activity against Gram-positive bacteria. Its efficacy against Gram-negative bacteria is limited, nonetheless it can be improved with the addition of surfactants, such as ethylenediaminetetraacetic acid (EDTA). The incorporation of peptide and other biomolecules within a biopolymer matrix provides protection maintaining their antimicrobial potential. Bacterial nanocellulose (BNC) has been widely used as wound dressings. Its impressive water retention capacity (> 99 %) and porosity are beneficial to manage wounds due to its potential to absorb exudates, providing a breathable and humid environment. In this work, the functionalization of BNC with Nisin Z (BNC-NZ) via vacuum filtration is reported. The entrapment of the peptide inside the BNC films was confirmed through morphological characterization using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectrometry. Typical absorbance peaks of Nisin Z are easily identifiable at 1647 cm-1 (amide group) and 1520 cm-1 (bending of primary amines). Thermal Gravimetric Analysis (TGA) suggested that Nisin Z did not interfere with the BNC matrix. The antimicrobial activity of Nisin Z against five of the most common bacteria found in burn wounds was verified by Minimum Bactericidal Concentration (MBC) ranging 8.0-256.0 µg/mL. Agar Diffusion and Shake Flask methods revealed the potential of BNC-NZ for prospective applications in burn wound dressings. | por |
dc.description.sponsorship | This work is financed by FEDER funds through COMPETE and by national funds through FCT via the projects POCI-01-0145-FEDER-028074 and UID/CTM/00264/2020. L.M. and T.D.T also acknowledge FCT for their PhD scholarships with references 2020.04919.BD and 2020.06046.BD. | por |
dc.language.iso | eng | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00264%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/2020.04919.BD/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/2020.06046.BD/PT | - |
dc.rights | openAccess | por |
dc.subject | Textile | por |
dc.subject | Antimicrobial peptides | por |
dc.subject | Bacterial nanocellulose | por |
dc.subject | Wound dressing | por |
dc.subject | Nisin Z | por |
dc.subject | Burn wounds | por |
dc.title | Antimicrobial activity of a bacterial nanocellulose film functionalized with Nisin Z for prospective burn wounds treatment | por |
dc.type | conferencePoster | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://sciforum.net/paper/view/12708 | por |
oaire.citationConferenceDate | 15 - 30 June 2022 | por |
sdum.event.title | The 2nd International Electronic Conference on Antibiotics-Drugs for Superbugs: Antibiotic Discovery, Modes of Action And Mechanisms of Resistance | por |
sdum.event.type | conference | por |
oaire.citationConferencePlace | Online | por |
dc.subject.fos | Engenharia e Tecnologia::Engenharia dos Materiais | por |
Aparece nas coleções: | DET/2C2T - Comunicações em congressos internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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slides.pdf | 1,62 MB | Adobe PDF | Ver/Abrir |