Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/81037
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Pastrana, Cristina | por |
dc.contributor.author | Guerreiro, Joana | por |
dc.contributor.author | Elumalai, Monisha | por |
dc.contributor.author | Carpena-Torres, Carlos | por |
dc.contributor.author | Crooke, Almudena | por |
dc.contributor.author | Carracedo, Gonzalo | por |
dc.contributor.author | Prado, Marta | por |
dc.contributor.author | Huete-Toral, Fernando | por |
dc.date.accessioned | 2022-12-09T09:45:07Z | - |
dc.date.available | 2022-12-09T09:45:07Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Pastrana, C.; Guerreiro, J.R.L.; Elumalai, M.; Carpena-Torres, C.; Crooke, A.; Carracedo, G.; Prado, M.; Huete-Toral, F. Dual-Mode Gold Nanoparticle-Based Method for Early Detection of Acanthamoeba. Int. J. Mol. Sci. 2022, 23, 14877. https://doi.org/10.3390/ijms232314877 | por |
dc.identifier.isbn | 1424-6783 | - |
dc.identifier.issn | 1661-6596 | por |
dc.identifier.uri | https://hdl.handle.net/1822/81037 | - |
dc.description.abstract | Acanthamoeba keratitis is an aggressive and rapidly progressing ocular pathology whose main risk factor is the use of contact lenses. An early and differential diagnosis is considered the main factor to prevent the progression and improve the prognosis of the pathology. However, current diagnosis techniques require time, complex and costly materials making an early diagnosis challenging. Thus, there is a need for fast, accessible, and accurate methods for Acanthamoeba detection by practitioners for timely and suitable treatment and even for contact lens user as preventive diagnosis. Here, we developed a dual-mode colorimetric-based method for fast, visual, and accurate detection of Acanthamoeba using gold nanoparticles (AuNPs). For this strategy, AuNPs were functionalized with thiolated probes and the presence of target Acanthamoeba genomic sequences, produce a colorimetric change from red to purple. This approach allows the detection of 0.02 and 0.009 μM of the unamplified Acanthamoeba genome by the naked eye in less than 20 min and by color analysis using a smartphone. Additionally, real samples were successfully analyzed showing the potential of the technology considering the lack of point-of-care tools that are mostly needed. | por |
dc.description.sponsorship | This work was supported by an industrial project (ND2017/BMD7676) granted by the community of Madrid. | por |
dc.language.iso | eng | por |
dc.publisher | MDPI | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | Acanthamoeba | por |
dc.subject | Acanthamoeba keratitis | por |
dc.subject | gold nanoparticles | por |
dc.subject | colorimetric assay | por |
dc.subject | color coordinates | por |
dc.subject | optical detection | por |
dc.title | Dual-mode gold nanoparticle-based method for early detection of Acanthamoeba | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/23/23/14877 | por |
dc.comments | CEB55949 | por |
oaire.citationStartPage | 14877 | por |
oaire.citationIssue | 23 | por |
oaire.citationConferencePlace | Switzerland | - |
oaire.citationVolume | 23 | por |
dc.date.updated | 2022-12-08T13:11:14Z | - |
dc.identifier.eissn | 1422-0067 | por |
dc.identifier.doi | 10.3390/ijms232314877 | por |
dc.identifier.pmid | 36499204 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | International Journal of Molecular Sciences | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55949_1.pdf | 2,56 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons