Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/81138
Título: | Controlling Macrophage Polarization to Modulate Inflammatory Cues Using Immune-Switch Nanoparticles |
Autor(es): | Almeida, A. F. Miranda, M. S. Vinhas, Carla Adriana Araújo Gonçalves, A I Gomes, Manuela E. Rodrigues, Márcia T. |
Palavras-chave: | cytokines Inflammation macrophages magnetically assisted technologies SPION targeted delivery |
Data: | Dez-2022 |
Editora: | MDPI |
Revista: | International Journal of Molecular Sciences |
Citação: | Almeida A. F., Miranda M. S., Vinhas A., Gonçalves A. I., Gomes M. E., Rodrigues M. T. Controlling Macrophage Polarization to Modulate Inflammatory Cues Using Immune-Switch Nanoparticles, International Journal o f Molecular Sciences, Vol. 23, Issue 23, doi:10.3390/ijms232315125, 2022 |
Resumo(s): | The persistence of inflammatory mediators in tissue niches significantly impacts regenerative outcomes and contributes to chronic diseases. Interleukin-4 (IL4) boosts pro-healing phenotypes in macrophages (MÏ ) and triggers the activation of signal transducer and activator of transcription 6 (STAT6). Since the IL4/STAT6 pathway reduces MÏ responsiveness to inflammation in a targeted and precise manner, IL4 delivery offers personalized possibilities to overcome inflammatory events. Despite its therapeutic potential, the limited success of IL4-targeted delivery is hampered by inefficient vehicles. Magnetically assisted technologies offer precise and tunable nanodevices for the delivery of cytokines by combining contactless modulation, high tissue penetration, imaging features, and low interference with the biological environment. Although superparamagnetic iron oxide nanoparticles (SPION) have shown clinical applicability in imaging, SPION-based approaches have rarely been explored for targeted delivery and cell programming. Herein, we hypothesized that SPION-based carriers assist in efficient IL4 delivery to MÏ , favoring a pro-regenerative phenotype (M2Ï ). Our results confirmed the efficiency of SPION-IL4 and MÏ responsiveness to SPION-IL4 with evidence of STAT6-mediated polarization. SPION-IL4-treated MÏ showed increased expression of M2Ï associated-mediators (IL10, ARG1, CCL2, IL1Ra) when compared to the well-established soluble IL4. The ability of SPION-IL4 to direct MÏ polarization using sophisticated magnetic nanotools is valuable for resolving inflammation and assisting innovative strategies for chronic inflammatory conditions. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/81138 |
DOI: | 10.3390/ijms232315125 |
ISSN: | 1661-6596 |
e-ISSN: | 1422-0067 |
Versão da editora: | https://www.mdpi.com/1422-0067/23/23/15125 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20869-ijms-23-15125-v2-1.pdf | 2,12 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons