Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/85220
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Pozdniakova, T. A. | por |
dc.contributor.author | Cruz, João P. | por |
dc.contributor.author | Silva, Paulo César | por |
dc.contributor.author | Azevedo, Flávio | por |
dc.contributor.author | Parpot, Pier | por |
dc.contributor.author | Domingues, Maria Rosario | por |
dc.contributor.author | Carlquist, Magnus | por |
dc.contributor.author | Johansson, Björn | por |
dc.date.accessioned | 2023-06-27T10:00:08Z | - |
dc.date.available | 2023-06-27T10:00:08Z | - |
dc.date.issued | 2023-12 | - |
dc.identifier.citation | Pozdniakova, T. A.; Cruz, J. P.; Silva, P. C.; Azevedo, F.; Parpot, Pier; Domingues, M. R.; Carlquist, M.; Johansson, B., Optimization of a hybrid bacterial/Arabidopsis thaliana fatty acid synthase system II in Saccharomyces cerevisiae. Metabolic Engineering Communications, 17(e00224), 2023 | por |
dc.identifier.issn | 2214-0301 | por |
dc.identifier.uri | https://hdl.handle.net/1822/85220 | - |
dc.description.abstract | Fatty acids are produced by eukaryotes like baker’s yeast Saccharomyces cerevisiae mainly using a large multifunctional type I fatty acid synthase (FASI) where seven catalytic steps and a carrier domain are shared between one or two protein subunits. While this system may offer efficiency in catalysis, only a narrow range of fatty acids are produced. Prokaryotes, chloroplasts and mitochondria rely instead on a FAS type II (FASII) where each catalytic step is carried out by a monofunctional enzyme encoded by a separate gene. FASII is more flexible and capable of producing a wider range of fatty acid structures, such as the direct production of unsaturated fatty acids. An efficient FASII in the preferred industrial organism S. cerevisiae could provide a platform for developing sustainable production of specialized fatty acids. We functionally replaced either yeast FASI genes (FAS1 or FAS2) with a FASII consisting of nine genes from Escherichia coli (acpP, acpS and fab -A, -B, -D, -F, -G, -H, -Z) as well as three from Arabidopsis thaliana (MOD1, FATA1 and FATB). The genes were expressed from an autonomously replicating multicopy vector assembled using the Yeast Pathway Kit for in-vivo assembly in yeast. Two rounds of adaptation led to a strain with a maximum growth rate (μmax) of 0.19 h− 1 without exogenous fatty acids, twice the growth rate previously reported for a comparable strain. Additional copies of the MOD1 or fabH genes resulted in cultures with higher final cell densities and three times higher lipid content compared to the control. | por |
dc.description.sponsorship | This work was supported by the Fundação para a Ciência e Tecnologia Portugal (FCT) through Project FatVal POCI-01- 0145-FEDER-032506; CBMA was supported by the strategic program UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569) funded by national funds through the FCT I.P. and by the ERDF through the COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier B.V. | por |
dc.relation | POCI-01- 0145-FEDER-032506 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIA%2F04050%2F2013/PT | por |
dc.rights | openAccess | por |
dc.subject | Saccharomyces cerevisiae | por |
dc.subject | E. coli | por |
dc.subject | Fatty acid synthase | por |
dc.subject | FASI | por |
dc.subject | FASII | por |
dc.subject | Metabolic engineering | por |
dc.title | Optimization of a hybrid bacterial/Arabidopsis thaliana fatty acid synthase system II in Saccharomyces cerevisiae | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/metabolic-engineering-communications | por |
dc.comments | CEB56290 | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 10 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationVolume | 17 | por |
dc.date.updated | 2023-06-27T09:13:21Z | - |
dc.identifier.doi | 10.1016/j.mec.2023.e00224 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.journal | Metabolic Engineering Communications | por |
dc.identifier.articlenumber | e00224 | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56290_1.pdf | 1,68 MB | Adobe PDF | Ver/Abrir |