Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/86830
Título: | Squaraine dyes derived from indolenine and benzo[e]indole as potential fluorescent probes for HSA detection and antifungal agents |
Autor(es): | Gomes, Vanessa Sofia Dias Ferreira, João C. C. Boto, Renato E. F. Almeida, Paulo Fernandes, Jose R. Sousa, Maria João Gonçalves, M. Sameiro T. Reis, Lucinda V. |
Palavras-chave: | Squaraine dyes Photostability Photodynamic activity Fluorescence probes Human Serum Albumin |
Data: | 2022 |
Editora: | American Society for Photobiology |
Revista: | Photochemistry and Photobiology |
Citação: | V. S. D. Gomes, J. C. C. Ferreira, R E. F. Boto, P. Almeida, J. R. Fernandes, M. J. Sousa, M. S. T. Gonçalves, L. V. Reis, "Squaraine dyes derived from indolenine and benzo[e]indole as potential fluorescent probes for HSA detection and antifungal agents”, Photochemistry and Photobiology, 2022, 98(6), 1402–1417. |
Resumo(s): | Four squaraine dyes derived from 2,3,3-trimethylindolenine and 1,1,2-trimethyl-1H-benzo[e]indole with different combinations of barbituric groups attach to the central ring, having ester groups and alkyl chains in the nitrogen atoms of heterocyclic rings were synthesized. These dyes were fully characterized and their photophysical behavior was studied in ethanol and phosphate-buffered saline solution. Absorption and emission bands between 631 and 712 nm were detected, with the formation of aggregates in aqueous media, which is typical of this class of dyes. Tests carried out with 1,3-diphenylisobenzofuran allowed us to verify the ability of the dyes to produce singlet oxygen. The interaction of synthesized dyes with human serum albumin (HSA) was also evaluated, being demonstrated a linear correlation between fluorescence intensity and protein concentration. The antifungal potential of the dyes against the yeast Saccharomyces cerevisiae was evaluated using a broth microdilution assay. In order to test the photosensitizing capacity of the synthesized dyes, tests were carried out in the dark and with irradiation, using a custom-built light-emitting diode that emits close to the absorption wavelength of the studied dyes. The results showed that the interaction of dyes with HSA and the antifungal activity depends on the different structural modifications of the dyes. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/86830 |
DOI: | 10.1111/php.13624 |
ISSN: | 0031-8655 |
e-ISSN: | 1751-1097 |
Versão da editora: | https://onlinelibrary.wiley.com/doi/full/10.1111/php.13624 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CDQuim - Artigos (Papers) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Manuscript revision-1.pdf | 1,62 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons