Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/88381
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Simões, Lúcia Chaves | por |
dc.contributor.author | Oliveira, Isabel | por |
dc.contributor.author | Borges, Anabela | por |
dc.contributor.author | Gomes, Inês Bezerra | por |
dc.contributor.author | Simões, Manuel | por |
dc.date.accessioned | 2024-01-31T16:14:28Z | - |
dc.date.available | 2024-01-31T16:14:28Z | - |
dc.date.issued | 2023-12 | - |
dc.identifier.citation | Simões, L. C., Oliveira, I., Borges, A., Gomes, I. B., & Simões, M. (2023, December 14). A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks. (L. J. MacDonald, Ed.), Journal of Microbiology & Biology Education. American Society for Microbiology. http://doi.org/10.1128/jmbe.00099-23 | por |
dc.identifier.issn | 1935-7877 | por |
dc.identifier.uri | https://hdl.handle.net/1822/88381 | - |
dc.description | The following material is available online at: https://journals.asm.org/doi/10.1128/jmbe.00099-23 | por |
dc.description.abstract | Shake flask cultivation is a routine technique in microbiology and biotechnology laboratories where cell growth can be affected by the hydrodynamic conditions, which depend on the agitation velocity, shaking diameter, and shake flask size. Liquid agitation is implemented inherently to increase aeration, substrate transfer to the cells, and prevent sedimentation, disregarding the role of hydrodynamics in microbial growth and metabolism. Here, we present a simple approach to help standardize the hydrodynamic forces in orbital shakers to increase the experimental accuracy and reproducibility and give students a better knowledge of the significance of the agitation process in microbial growth. | por |
dc.description.sponsorship | This research was funded by LA/P/0045/2020 (ALiCE), UIDB/00511/2020, and UIDP/00511/2020 (LEPABE), funded by national funds through FCT/MCTES (PIDDAC); UIDB/04469/2020 (CEB) and LABBELS—Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020; Germirrad-POCI-01–0247-FEDER-072237, funded by FEDER funds through COMPETE2020–Programa Operacional Competitividade e Internacionalização (POCI) and by national funds (PIDDAC) through FCT/MCTES. | por |
dc.language.iso | eng | por |
dc.publisher | American Society for Microbiology | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00511%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00511%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | por |
dc.subject | agitation | por |
dc.subject | hydrodynamic force | por |
dc.subject | microbial growth | por |
dc.subject | shake flasks | por |
dc.subject | standardization | por |
dc.title | A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://journals.asm.org/doi/10.1128/jmbe.00099-23 | por |
dc.comments | CEB56574 | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 4 | por |
oaire.citationIssue | 3 | por |
oaire.citationVolume | 24 | por |
dc.date.updated | 2024-01-20T19:03:37Z | - |
dc.identifier.eissn | 1935-7885 | por |
dc.identifier.doi | 10.1128/jmbe.00099-23 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.journal | Journal of Microbiology & Biology Education | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56574_1.pdf | 165,43 kB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons