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https://hdl.handle.net/1822/88858
Título: | M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue |
Autor(es): | Martins, Ivone M. Lima, Alexandre de Graaff, Wim Cristóvão, Joana S. Brosens, Niek Aronica, Eleonora Kluskens, Leon Gomes, Cláudio M. Azeredo, Joana Kessels, Helmut W. |
Data: | 2024 |
Editora: | Springer Nature |
Revista: | Communications Biology |
Citação: | Martins, I.M., Lima, A., de Graaff, W. et al. M13 phage grafted with peptide motifs as a tool to detect amyloid-β oligomers in brain tissue. Commun Biol 7, 134 (2024). https://doi.org/10.1038/s42003-024-05806-5 |
Resumo(s): | Oligomeric clusters of amyloid- (A) are one of the major biomarkers for Alzheimers disease (AD). However, proficient methods to detect A-oligomers in brain tissue are lacking. Here we show that synthetic M13 bacteriophages displaying A-derived peptides on their surface preferentially interact with A-oligomers. When exposed to brain tissue isolated from APP/PS1-transgenic mice, these bacteriophages detect small-sized A-aggregates in hippocampus at an early age, prior to the occurrence of A-plaques. Similarly, the bacteriophages reveal the presence of such small A-aggregates in post-mortem hippocampus tissue of ADpatients. These results advocate bacteriophages displaying A-peptides as a convenient and low-cost tool to identify A-oligomers in post-mortem brain tissue of AD-model mice and AD patients. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/88858 |
DOI: | 10.1038/s42003-024-05806-5 |
ISSN: | 2399-3642 |
Versão da editora: | https://www.nature.com/articles/s42003-024-05806-5 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56602_1.pdf | 1,34 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons