Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/89189
Título: | Comparison of the adsorption of linear and cyclic antimicrobial peptides onto cellulosic compounds-reinforced poly(vinyl alcohol) films using QCM-D |
Autor(es): | Teixeira, Marta A. Murthy, N. Sanjeeva Ferreira, Diana P. Felgueiras, Helena Prado |
Palavras-chave: | PVA-based spin-coated films Cellulosic compounds reinforcement Peptide-surface affinity Adsorption kinetics Quartz crystal microbalance with dissipation monitoring |
Data: | 2024 |
Editora: | Elsevier 1 |
Revista: | Colloids and Surfaces A: Physicochemical and Engineering Aspects |
Resumo(s): | Understanding peptide adsorption kinetics onto biomaterial surfaces is crucial for developing wound treatments. This study aims to explore the influence of cellulose acetate (CA) and cellulose nanocrystals (CNC) on peptide adsorption via quartz crystal microbalance with dissipation monitoring (QCM-D), using a cyclic peptide, Tiger 17, and a linear, Pexiganan. PVA was reinforced with 10 and 20% w/v of CA and CNC, spin-coated onto QCM-D sensors, and crosslinked with glutaraldehyde. Films containing higher percentages of cellulosic compounds promoted the highest peptide adsorption, with CNC-containing films being the most effective. While C80/20 PVA/CNC films achieved adsorption masses of ≈199 and ≈150 ng/cm2 for Tiger 17 and Pexiganan, respectively, the C80/20 PVA/CA films attained ≈168 and ≈122 ng/cm2 . The peptides’ structure also influenced adsorption, with Tiger 17 reaching greater frequency drops (ΔF) than Pexiganan. Sequential adsorption studies corroborated these findings. Even though the tendency was for PVA/CNC to promote the highest peptide binding, it was the PVA/CA films that reached the greatest peptide loading amount with the sequence Pexiganan + Tiger 17. Data are encouraging for developing new wound therapies reinforced with cellulosic compounds and modified with Tiger 17 and Pexiganan. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/89189 |
DOI: | 10.1016/j.colsurfa.2024.133490 |
ISSN: | 0927-7757 |
e-ISSN: | 1873-4359 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0927775724003510?via%3Dihub |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2024 Colloids and Surfaces A_Marta A.pdf | 4,92 MB | Adobe PDF | Ver/Abrir |