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https://hdl.handle.net/1822/9094
Título: | Production of 3-hydroxy-γ-decalactone, the precursor of two decenolides with flavouring properties, by the yeast Yarrowia lipolytica |
Autor(es): | Escamilla Garcia, Erandi Aguedo, Mário Gomes, Nelma Choquet, Armelle Belo, Isabel Teixeira, J. A. Belin, Jean-Marc Waché, Yves |
Palavras-chave: | 3-Hydroxy-γ-decalactone β-Oxidation fluxes Oxygen Yarrowia lipolytica 3-Hydroxy-gamma-decalactone beta-Oxidation fluxes |
Data: | Mai-2009 |
Editora: | Elsevier B.V. |
Revista: | Journal of Molecular Catalysis B: Enzymatic |
Citação: | "Journal of Molecular Catalysis B : Enzymatic". ISSN 1381-1177. 57:1-4 (May 2009) 22-26. |
Resumo(s): | 3-Hydroxy-γ-decalactone is the precursor of dec-2 and dec-3-en-4-olides which are valuable aroma compounds not yet produced. To promote the accumulation of this lactone, the yeast Yarrowia lipolytica was placed in different environmental conditions aiming at altering β-oxidation fluxes. The concentration of substrate, pH, aeration and dissolved oxygen level were modified. We observed an important accumulation at low aeration (0.40 molar yields) and, to a lesser extent, at lower pH (0.15). As oxygen played a key-role, we evaluated its effect at fixed dissolved oxygen and at the pH which was the most favourable to the biotransformation (pH 4.5). At 5% and 30% dissolved oxygen, yields reached 0.50. β-Oxidation fluxes are very dependent on the presence of oxygen and conditions of accumulation of 3-hydroxy-γ-decalactone with very high yields were identified. These results are an important step in the production of the two decenolides. Moreover, they show the high dependence of β-oxidation fluxes on environmental conditions and relate these conditions to the accumulation of intermediates, results that are of interest to all the processes using yeast on lipids or alkanes. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/9094 |
DOI: | 10.1016/j.molcatb.2008.06.010 |
ISSN: | 1381-1177 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Escamilla-Garcia_JMCE[1].pdf | 611,45 kB | Adobe PDF | Ver/Abrir |