Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/91465
Título: | Insulin nucleation kinetics in an oscillatory flow-based platform: Protein crystallization as a highly reproducible separation process |
Autor(es): | Ferreira, Joan Araújo, Sofia Ferreira, António Teixeira, J. A. Campos, João Moreira de Rocha, Fernando Castro, Filipa Juliana Fernandes |
Palavras-chave: | Insulin Nucleation kinetics Crystallization Oscillatory flow reactor |
Data: | Mar-2024 |
Editora: | Institute of Chemical Engineers |
Revista: | Chemical Engineering Research & Design |
Citação: | Ferreira, Joana; Araújo, Sofia; Ferreira, António; Teixeira, José A.; Campos, João Moreira de; Rocha, Fernando; Castro, Filipa, Insulin nucleation kinetics in an oscillatory flow-based platform: Protein crystallization as a highly reproducible separation process. Chemical Engineering Research & Design, 203, 453-466, 2024 |
Resumo(s): | Insulin is a human therapeutic protein that has been used to treat diabetes for almost a century. Its crystallization has been of significant interest since the protein is commonly administered by subcutaneous injections of crystalline formulations, where crystal size distribution (CSD) has a critical role in product release and injectability. Herein, insulin crystallization was investigated in a unique platform based on oscillatory flow mixing technology. Assays were carried out at different supersaturation ratios (i.e., insulin concentrations) in the presence and absence of acetone, and turbidity of the crystallization solution was monitored over time by UV-Vis spectrophotometry. The results show the impact of both supersaturation ratio and acetone on nucleation kinetics, as well as on CSD and growth rate. As the initial supersaturation increases, the nucleation rate increases, and the growth rate and mean crystal size decrease. The presence of acetone allows a faster nucleation event, a narrower CSD and a larger mean crystal size. The kinetic parameter A derived from the Classical Nucleation Theory (CNT) also indicates the kinetics of molecular attachment acceleration in the presence of acetone. These findings contribute to a better understanding of insulin crystallization processes under oscillatory flow. Thus, the described strategy and oscillatory flow-based systems are very promising for optimizing protein crystallization processes to be used during the downstream separation of bioproducts. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/91465 |
DOI: | 10.1016/j.cherd.2024.01.057 |
ISSN: | 0263-8762 |
Versão da editora: | http://www.journals.elsevier.com/chemical-engineering-research-and-design/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_57750_1.pdf | 2,14 MB | Adobe PDF | Ver/Abrir |