Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/91468
Título: | Non-Mature miRNA-encoded micropeptide miPEP166c stimulates anthocyanin and proanthocyanidin synthesis in grape berry cells |
Autor(es): | Vale, Mariana Badim, Hélder Gerós, H. Conde, Artur Jorge Silva |
Palavras-chave: | Non-mature miRNA-encoded micropeptides (miPEPs) Secondary metabolism Anthocyanins Proanthocyanidins Grape berry cells |
Data: | 26-Jan-2024 |
Editora: | MDPI Open Access Publishing, |
Revista: | International Journal of Molecular Sciences |
Citação: | Vale, Mariana; Badim, Hélder; Gerós, Hernâni; Conde, Artur, Non-Mature miRNA-encoded micropeptide miPEP166c stimulates anthocyanin and proanthocyanidin synthesis in grape berry cells. International Journal of Molecular Sciences, 25(3), 1539, 2024 |
Resumo(s): | The phenylpropanoid and flavonoid pathways exhibit intricate regulation, not only influenced by environmental factors and a complex network of transcription factors but also by post-transcriptional regulation, such as silencing by microRNAs and miRNA-encoded micropeptides (miPEPs). VviMYBC2-L1 serves as a transcriptional repressor for flavonoids, playing a crucial role in coordinating the synthesis of anthocyanin and proanthocyanidin. It works in tandem with their respective transcriptional activators, VviMYBA1/2 and VviMYBPA1, to maintain an equilibrium of flavonoids. We have discovered a miPEP encoded by miR166c that appears to target VviMYBC2-L1. We conducted experiments to test the hypothesis that silencing this transcriptional repressor through miPEP166c would stimulate the synthesis of anthocyanins and proanthocyanidins. Our transcriptional analyses by qPCR revealed that the application of exogenous miPEP166c to Gamay Fréaux grape berry cells resulted in a significant upregulation in flavonoid transcriptional activators (VviMYBA1/2 and VviMYBPA1) and structural flavonoid genes (VviLDOX and VviDFR), as well as genes involved in the synthesis of proanthocyanidins (VviLAR1 and VviANR) and anthocyanins (VviUFGT1). These findings were supported by the increased enzyme activities of the key enzymes UFGT, LAR, and ANR, which were 2-fold, 14-fold, and 3-fold higher, respectively, in the miPEP166c-treated cells. Ultimately, these changes led to an elevated total content of anthocyanins and proanthocyanidins. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/91468 |
ISBN: | 1424-6783 |
DOI: | 10.3390/ijms25031539 |
ISSN: | 1422-0067 |
Versão da editora: | http://www.mdpi.com/journal/ijms |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_57747_1.pdf | 3,65 MB | Adobe PDF | Ver/Abrir |