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https://hdl.handle.net/1822/91946
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Campo DC | Valor | Idioma |
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dc.contributor.author | Lüdtke, Fernanda Luisa | por |
dc.contributor.author | Fernandes, Jean Michel | por |
dc.contributor.author | Gonçalves, Raquel Filipa Silva | por |
dc.contributor.author | Martins, Joana T. | por |
dc.contributor.author | Berni, Paulo | por |
dc.contributor.author | Ribeiro, Ana P. B. | por |
dc.contributor.author | Vicente, A. A. | por |
dc.contributor.author | Pinheiro, A. C. | por |
dc.date.accessioned | 2024-06-20T15:31:21Z | - |
dc.date.available | 2024-06-20T15:31:21Z | - |
dc.date.issued | 2024-06 | - |
dc.identifier.citation | Lüdtke, Fernanda Luisa; Fernandes, J. -M.; Gonçalves, Raquel F. S.; Martins, Joana T.; Berni, P.; Ribeiro, Ana P. B.; Vicente, António A.; Pinheiro, Ana Cristina, Performance of -carotene-loaded nanostructured lipid carriers under dynamic in vitro digestion system: Influence of the emulsifier type. Journal of Food Science, 89(6), 3290-3305, 2024 | por |
dc.identifier.issn | 0022-1147 | por |
dc.identifier.uri | https://hdl.handle.net/1822/91946 | - |
dc.description.abstract | A better understanding of how emulsifier type could differently influence the behavior of nanostructured lipid carriers (NLC) under the gastrointestinal digestion process, as well as at the cellular level, is of utmost importance for the NLC-based formulations optimization and risk assessment in the food field. In this study, NLC composed by fully hydrogenated soybean and high-oleic sunflower oils were prepared using soy lecithin (NLC L) or Tween 80 (NLC T) as an emulsifier. -Carotene was entrapped within NLC developed as a promising strategy to overcome -carotene's low bioavailability and stability. The effect of emulsifier type on the digestibility of -carotene-loaded NLC was evaluated using an in vitro dynamic digestion model mimicking peristalsis motion. The influence of -carotene-loaded NLC on cell viability was assessed using Caco-2 cells in vitro. NLC T remained stable in the gastric compartment, presenting particle size (PS) similar to the initial NLC (PS: 245.68 and 218.18 nm, respectively), while NLC L showed lower stability (PS > 1000 nm) in stomach and duodenum phases. NLC T also provided high -carotene protection and delivery capacity (i.e., -carotene bioaccessibility increased 10-fold). Based on the results of digestion studies, NLC T has shown better physical stability during the passage through the in vitro dynamic gastrointestinal system than NLC L. Moreover, the developed NLC did not compromise cell viability up to 25 µg/mL of -carotene. Thus, the NLC developed proved to be a biocompatible structure and able to incorporate and protect -carotene for further food applications. | por |
dc.description.sponsorship | F.L.L. and P.B. acknowledge the Sao Paulo Research Foundation (FAPESP) for the financial support (grants #2018/03172-0, #2019/05176-6, #2019/27354-3, and #2021/02271-8). F.L.L. also thanks Iceland Liechten-stein Norway Grants (PT-INNOVATION-0105) for the fellowship. J.-M.F. and R.G. acknowledge the Foundation for Science and Technology (FCT) for their fellowships (SFRH/BD/147286/2019 and SFRH/BD/140182/2018, respectively). J.T.M. acknowledges FCT for her Assistant Research contract obtained under the scope of Scientific Stimulus Employment with reference 2022.00788.CEECIND/CP1718/CT0024 (https://doi.org/10. 54499/2022.00788.CEECIND/CP1718/CT0024). This study was supported by FCT under the scope of the strategic funding of UIDB/04469/2020 unit and by LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechnaical Systems, LA/P/0029/2020. | por |
dc.language.iso | eng | por |
dc.publisher | Wiley-Blackwell | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F147286%2F2019/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F140182%2F2018/PT | por |
dc.relation | 2022.00788.CEECIND/CP1718/CT0024 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.relation | LA/P/0029/2020 | por |
dc.rights | openAccess | por |
dc.subject | Carotenoids | por |
dc.subject | Cell viability | por |
dc.subject | Dynamic in vitro gastrointestinal system | por |
dc.subject | Lipid nanoparticles | por |
dc.title | Performance of β-carotene-loaded nanostructured lipid carriers under dynamic in vitro digestion system: influence of the emulsifier type | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://ift.onlinelibrary.wiley.com/journal/17503841 | por |
dc.comments | CEB57856 | por |
oaire.citationStartPage | 3290 | por |
oaire.citationEndPage | 3305 | por |
oaire.citationIssue | 6 | por |
oaire.citationConferencePlace | United States | - |
oaire.citationVolume | 89 | por |
dc.date.updated | 2024-06-17T20:54:28Z | - |
dc.identifier.eissn | 1750-3841 | por |
dc.identifier.doi | 10.1111/1750-3841.17113 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.journal | Journal of Food Science | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
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document_57856_1.pdf | 999,63 kB | Adobe PDF | Ver/Abrir |