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https://hdl.handle.net/1822/10369
Título: | “On the collander equation”: protein partitioning in polymer/polymer aqueous two-phase systems |
Autor(es): | Madeira, Pedro Teixeira, J. A. Macedo, Eugénia Mikheeva, Larissa M. Zaslavsky, Boris Y. |
Palavras-chave: | Aqueous two-phase systems Protein partitioning Collander equation |
Data: | Mai-2008 |
Editora: | Elsevier 1 |
Revista: | Journal of Chromatography A |
Citação: | "Journal of Chromatography A". ISSN 0021-9673. 1190:1-2 (May 2008) 39-43. |
Resumo(s): | Distribution coefficients of randomly selected proteins were measured in aqueous two-phase systems (ATPSs) formed by different combinations of Dextran-75 (Dex), Ficoll-70, polyethylene glycol-8000 (PEG), hydroxypropyl starch-100 (PES), and Ucon50HB5100 (Ucon, a random copolymer of ethylene glycol and propylene glycol) at particular polymer concentrations, all containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4. Most of the proteins in the PEG-Ucon system precipitated at the interface. In the other ATPSs, namely, PES-PEG, PES-Ucon, Ficoll-PEG, Ficoll-Ucon, and in Dex-PEG and Dex-Ucon described earlier the distribution coefficients for the proteins were correlated according to the solvent regression equation: ln Ki = aio ln Ko + bio, where Ki and Ko are the distribution coefficients for any protein in the ith and oth two-phase systems. Coefficients aio and bio are constants, the values of which depend upon the particular compositions of the two-phase systems under comparison. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/10369 |
DOI: | 10.1016/j.chroma.2008.03.003 |
ISSN: | 0021-9673 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2008-046.pdf | 375,2 kB | Adobe PDF | Ver/Abrir |