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https://hdl.handle.net/1822/11930| Título: | Fractionation and recovery of whey proteins by hydrophobic interaction chromatography |
| Autor(es): | Santos, Maria João Teixeira, J. A. Rodrigues, L. R. |
| Palavras-chave: | Hydrophobic interaction chromatography whey proteins b-lactoglobulin recovery & isolation Recovery Isolation β-Lactoglobulin |
| Data: | 22-Mar-2011 |
| Editora: | Elsevier 1 |
| Revista: | Journal of Chromatography B |
| Citação: | "Journal of Chromatography B". ISSN 1570-0232. 879 (2011) 475-479. |
| Resumo(s): | A method for the recovery and fractionation of whey proteins from a whey protein concentrate (80%, w/w) by hydrophobic interaction chromatography is proposed. Standard proteins and WPC 80 dissolved in phosphate buffer with ammonium sulfate 1M were loaded in a HiPrep Octyl Sepharose FF column coupled to a fast protein liquid chromatography (FPLC) system and eluted by decreasing the ionic strength of the buffer using a salt gradient. The results showed that the most hydrophobic protein from whey is -lactalbumin and the less hydrophobic is lactoferrin. It was possible to recover 45.2% of -lactoglobulin using the HiPrep Octyl Sepharose FF column from the whey protein concentrate mixture with 99.6% purity on total protein basis |
| Tipo: | Artigo |
| URI: | https://hdl.handle.net/1822/11930 |
| DOI: | 10.1016/j.jchromb.2011.01.003 |
| ISSN: | 1570-0232 |
| Arbitragem científica: | yes |
| Acesso: | Acesso aberto |
| Aparece nas coleções: |
Ficheiros deste registo:
| Ficheiro | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| Santos_2011.pdf | Santos_2011 | 340,75 kB | Adobe PDF | Ver/Abrir |
