Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/11948
Título: | Characterization of galactooligosaccharides produced by β-galactosidase immobilized onto magnetized Dacron |
Autor(es): | Neri, D. F. M. Balcão, V. M. Cardoso, Susana M. Silva, Artur M. S. Domingues, M. R. M. Torres, D. Rodrigues, L. R. Carvalho Junior, L. B. Teixeira, J. A. |
Palavras-chave: | Galactooligosaccharides Immobilized b-galactosidase Dracon Magnetic support |
Data: | Mar-2011 |
Editora: | Elsevier 1 |
Revista: | International Dairy Journal |
Citação: | "International Dairy Journal." ISSN 0958-6946. 21:3 (Mar. 2011) 172-178. |
Resumo(s): | Bioconversions using enzymes immobilized in magnetic supports present significant advantages due to the easy separation of the enzyme from the reaction mixture and the simplicity and low cost of the support preparation. The characterization of the oligosaccharide mixture obtained by the action of b-galactosidase covalently attached, via glutaraldehyde, to a hydrazideeDacronemagnetite composite is presented. The fractionation of the oligosaccharide mixture by high performance liquid chromatography, followed by the analysis of the purified compounds by mass spectrometry and nuclear magnetic resonance spectroscopy permitted the identification of glucose, galactose, lactose and a hexose disaccharide containing a 1/6 linkage. Also, the following GOS were identified: b-D-Galp-(1/6)-b-D-Galp-(1/4)- Glcp, b-D-Galp-(1/4)-[b-D-Galp-(1/6)]-Glcp and b-D-Galp-(1/6)-b-D-Galp-(1/6)-b-D-Galp-(1/4)- Glcp. When GOS yield (26.2%) and kinetics of biotransformation of lactose by the Dacron immobilized b-galactosidase were compared with values obtained for the enzyme immobilized in other magnetic supports, similar behaviour was observed |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/11948 |
DOI: | 10.1016/j.idairyj.2010.10.009 |
ISSN: | 0958-6946 |
Versão da editora: | http://www.sciencedirect.com/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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INDA3157.pdf | Neri_2011 | 857,02 kB | Adobe PDF | Ver/Abrir |