Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/14856
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Campo DC | Valor | Idioma |
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dc.contributor.author | Abrunhosa, Luís | - |
dc.contributor.author | Calado, Thalita | - |
dc.contributor.author | Resende, Tiago | - |
dc.contributor.author | Venâncio, Armando | - |
dc.date.accessioned | 2011-12-06T11:57:31Z | - |
dc.date.available | 2011-12-06T11:57:31Z | - |
dc.date.issued | 2011-10 | - |
dc.identifier.issn | 1520-572X | por |
dc.identifier.issn | 1082-6076 | por |
dc.identifier.uri | https://hdl.handle.net/1822/14856 | - |
dc.description.abstract | The recent discovery that Aspergillus niger can produce fumonisins raises a new mycotoxigenic risk that requires additional studies to assess the impact on agricultural commodities. Here, we report the development and validation of a sensitive LC-fluorescence method for the identification of fumonisin B2 in black aspergilli cultures. The performances of naphthalene-2,3-- dicarboxaldehyde (NDA), o-phthaldialdehyde (OPA), and 9-fluorenylmethyl chloroformate (FMOC) for the precolumn derivatization of samples were evaluated and compared. The best methodology included the following elements: NDA was used during pre-column derivatization; the derivatives were separated by a 30min isocratic elution with acetonitrile=H2O=acetic acid (60:40:1, v=v=v), pumped at a flow rate of 1mL min 1 on a polar C18 reversed-phase YMC-Pack ODS-AQ column (250 4.6mm, 5 lm); and fluorescence detection using excitation and emission wavelengths of 420 and 500 nm, respectively. The calibration curve was found to give a good linear response (r2>0.9999) from 1 to 200 lg L 1. For FB2, which is the main fumonisin detected in black aspergilli thus far, this method measured an LOD and an LOQ of 2 and 6 lg kg 1, respectively. Using this method, the amount of FB2 in black aspergilli cultures can be simply and rapidly determined. | por |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) | por |
dc.language.iso | eng | por |
dc.publisher | Taylor & Francis | por |
dc.rights | restrictedAccess | por |
dc.subject | Aspergillus niger | por |
dc.subject | Fumonisin B2 | por |
dc.subject | black aspergilli | por |
dc.subject | fluorescence detection | por |
dc.subject | HPLC | por |
dc.subject | Naphthalene-2,3-dicarboxaldehyde | por |
dc.title | A fluorescence-LC method with NDA pre-column derivatization for fumonisin B2 determination in black aspergilli cultures | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://pubs.acs.org/doi/abs/10.1021/jf202123q | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 1594 | por |
oaire.citationEndPage | 1603 | por |
oaire.citationIssue | 15 | por |
oaire.citationTitle | Journal of Liquid Chromatography & Related Technologies | por |
oaire.citationVolume | 34 | por |
dc.identifier.doi | 10.1080/10826076.2011.575982 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Liquid Chromatography & Related Technologies | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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abrunhosa_etal_2011_JLCRT.pdf Acesso restrito! | documento principal | 289,58 kB | Adobe PDF | Ver/Abrir |