Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/1556
Título: | Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose |
Autor(es): | Domingues, Lucília Oliveira, Carla Cristina Marques de Castro, Inês Lima, Nelson Teixeira, J. A. |
Palavras-chave: | β-galactosidase production Flocculent yeast Extracellular protein production High-cell-density fermentation beta-galactosidase production |
Data: | 2004 |
Editora: | Society of Chemical Industry |
Revista: | Journal of Chemical Technology and Biotechnology |
Citação: | “Journal of chemical technology and biotechnology”. ISSN 0268-2575. 79 (2004) 809-815. |
Resumo(s): | Improved productivity and costs reduction in fermentation processes may be attained by using flocculating cell cultures. The production of extracellular heterologous β-galactosidase by recombinant flocculating Saccharomyces cerevisiae cells, expressing the lacA gene (coding for β-galactosidase) of Aspergillus niger under the ADHI promotor and terminator in a bioreactor was studied. The effects of lactose concentration and yeast extract concentration on β-galactosidase production in a semi-synthetic medium were analysed. The extracellular β-galactosidase activity increased linearly with increasing initial lactose concentrations (5–150 g dmˉ³ ). β-Galactosidase production also increased with increased yeast extract concentration. During the entire fermentation, no accumulation of the hydrolysed sugars, glucose and galactose, was observed. The catabolic repression of the recombinant strain when cultured in a medium containing equal amounts of glucose and galactose was confirmed. In complete anaerobiosis, the fermentation of lactose resulted in a very slow fermentation pattern with lower levels of β-galactosidase activity. The bioreactor operation together with optimisation of culture conditions (lactose and yeast extract concentration) led to a 21-fold increase in the extracellular β-galactosidase activity produced when compared with preliminary Erlenmeyer fermentations. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/1556 |
DOI: | 10.1002/jctb.1025 |
ISSN: | 0268-2575 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DCILM - Artigos (Papers) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
2004-18.pdf | 167,67 kB | Adobe PDF | Ver/Abrir |