Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/16068
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Campo DC | Valor | Idioma |
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dc.contributor.author | Coutinho, Isabel | - |
dc.contributor.author | Pereira, Clara | - |
dc.contributor.author | Pereira, Gil | - |
dc.contributor.author | Gonçalves, Jorge | - |
dc.contributor.author | Côrte-Real, Manuela | - |
dc.contributor.author | Saraiva, Lucília | - |
dc.date.accessioned | 2011-12-30T11:00:48Z | - |
dc.date.available | 2011-12-30T11:00:48Z | - |
dc.date.issued | 2011-05 | - |
dc.identifier.issn | 0014-4827 | por |
dc.identifier.uri | https://hdl.handle.net/1822/16068 | - |
dc.description.abstract | The role of individual protein kinase C (PKC) isoforms in the regulation of p53- mediated apoptosis is still uncertain. Using yeast cells co-expressing the human wild-type p53 and a single mammalian PKCa, d, e or z, we showed a differential regulation of p53- mediated apoptosis by these PKC isoforms. Whereas PKCa and z had no effect on p53 activity, PKCd and e stimulated a p53-mediated mitochondria-dependent apoptosis. Moreover, using pifithrin-a and -m, selective inhibitors of p53 transcriptional activity and mitochondrial p53 translocation, respectively, we showed the activation of a transcriptiondependent and -independent p53-mediated apoptosis by PKCd and e. The activation of mitochondrial p53 translocation by PKCd and e was further confirmed by immunofluorescence and Western blot analysis. Together, this work reveals the conservation in yeast of functional transcriptiondependent and -independent p53 apoptotic mechanisms. Furthermore, it gives mechanistic insights about the regulation of p53-mediated apoptosis by PKCd and e through modulation of p53 transcriptional activity and of its translocation to mitochondria. Finally, it underscores a major role of PKCd and e as positive regulators of p53-mediated apoptosis, and therefore as promising therapeutic targets in cancer. | por |
dc.description.sponsorship | REQUIMTE/CEQUP, FCT (I&D No 8/94; PTDC/ SAU-FAR/110848/2009), POCTI (QCA III), FEDER and U.Porto/ Santander Totta. I. Coutinho is recipient of a PhD fellowship from FCT (SFRH/BD/36066/2007). C. Pereira is recipient of a Post-Doctoral fellowship from FCT (SFRH/BPD/44209/2008). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/110848/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F36066%2F2007/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F44209%2F2008/PT | - |
dc.rights | openAccess | por |
dc.subject | P53 | por |
dc.subject | PKC isoforms | por |
dc.subject | Apoptosis | por |
dc.subject | Transcription | por |
dc.subject | Mitochondria | por |
dc.subject | Yeast | por |
dc.title | Distinct regulation of p53-mediated apoptosis by protein kinase calpha, delta, epsilon and zeta: evidence in yeast for transcription-dependent and -independent p53 apoptotic mechanisms | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 1083 | por |
oaire.citationEndPage | 1238 | por |
oaire.citationIssue | 8 | por |
oaire.citationTitle | Experimental Cell Research | por |
oaire.citationVolume | 317 | por |
dc.identifier.doi | 10.1016/j.yexcr.2011.02.007 | por |
dc.identifier.pmid | 21338602 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Experimental Cell Research | por |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Coutinho et al. 2011 post-print.pdf | post-print | 560,52 kB | Adobe PDF | Ver/Abrir |