Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/20661
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Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Garcia Cruz, Dunia M. | - |
dc.contributor.author | Gomes, Manuela E. | - |
dc.contributor.author | Reis, R. L. | - |
dc.contributor.author | Moratal, David | - |
dc.contributor.author | Salmerón-Sánchez, M. | - |
dc.contributor.author | Gómez Ribelles, J. L. | - |
dc.contributor.author | Mano, J. F. | - |
dc.date.accessioned | 2012-11-05T14:17:38Z | - |
dc.date.available | 2012-11-05T14:17:38Z | - |
dc.date.issued | 2010-12 | - |
dc.identifier.issn | 1549-3296 | por |
dc.identifier.uri | https://hdl.handle.net/1822/20661 | - |
dc.description.abstract | Bone Marrow mesenchymal stem cells can be induced to differentiate into osteoblasts to regenerate damaged bone tissue using tissue engineering techniques. In this study, we examine the use of chitosan scaffolds with double pore structure prepared by an innovative method that combines freeze gelation (that produces micropores) and particle leaching out technique (that produces interconnected spherical macropores) seeking to enhance the osteogenic differentiation of goat bone marrow stromal cells (GBMSCs). The double pore architecture of the scaffold was characterized by scanning electron microscopy (SEM), microcomputed tomography and confocal laser scanning microscopy. The obtained hierarchical pore structure allowed very efficient seeding of GBMSCs that are able to occupy the whole volume of the scaffold, showing good adhesion and proliferation. GBMSCs were differentiated into osteoblasts as indicated by alkaline phosphatase activity and osteocalcin expression. The results of this study demonstrate that chitosan scaffold may be promising biomaterial for bone regeneration. | por |
dc.description.sponsorship | Contract grant sponsor: Spanish Ministry of Education (including the FEDER financial support); contract grant number: MAT2007-66759-C03-01Contract grant sponsor: Science and Innovation Ministry; contract grant number: PTA2008-0943-PContract grant sponsor: Generalitat Valenciana (Grups d'investigacio Emergents); contract grant number: GV/2009/126Contract grant sponsors: VI National R&D&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions, Instituto de Salud Carlos III (European Regional Development Fund)Contract grant sponsor: FCT project; contract grant number: PTDC/QUI/68804/2006 | por |
dc.language.iso | eng | por |
dc.publisher | Wiley | por |
dc.rights | openAccess | por |
dc.subject | Chitosan | por |
dc.subject | Macroporous scaffolds | por |
dc.subject | Mesenchymal stem cells | por |
dc.subject | Tissue engineering | por |
dc.title | Differentiation of mesenchymal stem cells in chitosan scaffolds with double micro and macroporosity | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 1182 | por |
oaire.citationEndPage | 1193 | por |
oaire.citationIssue | 4 | por |
oaire.citationVolume | 95A | por |
dc.identifier.doi | 10.1002/jbm.a.32906 | por |
dc.identifier.pmid | 20925083 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Biomedical Materials Research: Part A | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |