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https://hdl.handle.net/1822/21991
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Firmino, A. D. G. | - |
dc.contributor.author | Gonçalves, M. Sameiro T. | - |
dc.date.accessioned | 2012-12-21T15:13:52Z | - |
dc.date.available | 2012-12-21T15:13:52Z | - |
dc.date.issued | 2012-07 | - |
dc.identifier.uri | https://hdl.handle.net/1822/21991 | - |
dc.description.abstract | In recent years, fluorescence techniques have seen an enormous improvement, being in nowadays an interesting tool in the visualization and quantification of biomolecules, frequently used in cell biology, biochemistry, proteomics, immunochemistry, flow cytometry and medical diagnostics.1 Although intrinsic fluorophores are of great interest, the development of new extrinsic fluorophores is crucial for the design of new fluorescent probes, namely with improved water solubility, functional groups, and enhanced fluorescence quantum efficiency at high emission wavelengths.2 As a continuation of our previous research,3 this communication describes the evaluation of absorption and emission properties of new mono- and bifunctionalised benzo[a]phenoxazinium chlorides 1a-c in ethanol, water, and at simulated physiological pH, focus on the variation of the concentration. In order to investigate the sensitivity of fluorescence of the long-wavelength fluorophores to pH, photophysical studies under controlled pH also were performed. | por |
dc.description.sponsorship | Fundação para a Ciência e Tecnologia (Portugal) - financial support through project PEst-C/QUI/UI0686/2011 (F-COMP-01-0124-FEDER-022716), FEDER-COMPETE, FCT-Portugal. The NMR spectrometer Bruker Avance III 400 is part of the National NMR Network and was acquired with funds from FCT and FEDER. | por |
dc.language.iso | eng | por |
dc.rights | restrictedAccess | por |
dc.subject | Benzo[a]phenoxazines | por |
dc.subject | Fluorescent probes | por |
dc.subject | Long-wavelength fluorophores | por |
dc.title | Absorption and fluorescence studies of new 3-amino-N-(2-hydroxy-, (3-hydroxypropoxy)- or (3-chloropropoxy)-5-(propylamino)-9H-benzo[a]phenoxazin-9-ylidene)propan-1-aminium chlorides | por |
dc.type | conferenceAbstract | - |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationConferenceDate | 08 jun. 2012 | por |
sdum.event.type | congress | por |
oaire.citationConferencePlace | Braga, Portugal | por |
oaire.citationTitle | II ETCAQ - II Encontro em Técnicas de Caracterização e Análise Química | por |
Aparece nas coleções: | CDQuim - Comunicações e Proceedings |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Abstract - II_ETCAQ- ADF.pdf Acesso restrito! | Final draft post-refereeing | 65,22 kB | Adobe PDF | Ver/Abrir |