Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/24347
Título: | Bioaccumulation of amylose-like glycans by Helicobacter pylori |
Autor(es): | Ferreira, José A. Pires, Cristiana Paulo, Marina Azevedo, N. F. Domingues, M. R. M. Vieira, M. J. Monteiro, Mario A. Coimbra, Manuel A. |
Palavras-chave: | Helicobacter pylori Cell-surface polysaccharide Glucan Environmental pressure |
Data: | 2009 |
Editora: | Wiley-Blackwell |
Revista: | Helicobacter |
Resumo(s): | Background: Helicobacter pylori cell surface is composed of lipopolysaccharides (LPSs) yielding structures homologous to mammalian Lewis O-chains blood group antigens. These structures are key mediators in the definition of host-microbial interactions and known to change their expression pattern in response to environmental pressure. Aims: The present work is focused on the identification of new H. pylori cell-surface glycosides. Special attention is further devoted to provide insights on the impact of in vitro subcultivation on H. pylori cell-surface phenotypes. Methods: Cell-surface glycans from H. pylori NCTC 11637 and two clinical isolates were recovered from the aqueous phase resulting from phenol:water extraction of intact bacteria. They were evaluated in relation to their sugars and glycosidic-linkages composition by CG-MS, size-exclusion chromatography, NMR, and Mass Spectrometry. H. pylori glycan profile was also monitored during subcultivation in vitro in agar and F12 liquid medium. Results: All three studied strains produce LPS expressing Lewis epitopes and express bioaccumulate amylose-like glycans. Bioaccumulation of amylose was found to be enhanced with the subcultivation of the bacterium on agar medium and accompanied by a decrease in the expression of LPS O-chains. In contrast, during exponential growth in F12 liquid medium, an opposite behavior is observed, that is, there is an increase in the overall amount of LPS and decrease in amylose content. Conclusions: This work shows that under specific environmental conditions, H. pylori expresses a phase-variable cell-surface α-(1→4)-glucose moiety. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/24347 |
DOI: | 10.1111/j.1523-5378.2009.00725.x |
ISSN: | 1083-4389 |
e-ISSN: | 1523-5378 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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j.1523-5378.2009.00725.x.pdf | 559,88 kB | Adobe PDF | Ver/Abrir |