Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/2877
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Rocha, I. | - |
dc.contributor.author | Ferreira, Eugénio C. | - |
dc.date.accessioned | 2005-09-13T09:22:29Z | - |
dc.date.available | 2005-09-13T09:22:29Z | - |
dc.date.issued | 2002 | - |
dc.identifier.citation | CAMACHO, E. F. ; BASAŇEZ, L. ; PUENTE, J. A. de la, ed. lit. – “Proceedings of the 15th IFAC world congress on automatic control, Barcelona, Spain, July 21-26, 2002”. Oxford : Pergamon Press, 2003. ISBN 0-08-044232-3. vol. Q. p. 461-466. | eng |
dc.identifier.isbn | 0-08-044232-3 | - |
dc.identifier.issn | 1474-6670 | por |
dc.identifier.uri | https://hdl.handle.net/1822/2877 | - |
dc.description.abstract | A model-based adaptive linearizing control law was derived for the regulation of the acetate concentration during the fed-batch fermentation of recombinant proteins with high cell density culture of Escherichia coli growing on glucose. An unstructured model for the growth was applied to the major metabolic pathways: oxidative growth on glucose, fermentative growth on glucose, oxidative growth on acetate, and maintenance. A model order reduction method was used to allow the development of the control algorithm without the knowledge of the kinetic structure being necessary. The non-linear model was subjected to transformations in order to obtain a linear behaviour for the control loop when a non-linear control is applied. The control law requires on-line acetate and carbon dioxide and oxygen transfer rates measurements. Acetate measurements are achieved with a developed Flow Injection Analysis (FIA) physical-chemical method. The gas transfer rates are calculated from gas analysis data obtained with a Mass Spectrometer (MS) connected to the exhaust gas line of the fermenter and also to the inlet aeration line. These calculations, as well as the implementation of the control law were performed through a MATLAB script embedded in a LABView program that also acquired data from the FIA system and other relevant state variables from the fermenter Digital Control Unit. Copyright 2002 IFAC | eng |
dc.description.sponsorship | Agência de Inovação - PROTEXPRESS. | eng |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) – PRAXIS XXI/BD/16961/98. | - |
dc.language.iso | eng | eng |
dc.publisher | International Federation of Automatic Control (IFAC) | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POCTI/PRAXIS XXI%2FBD%2F16961%2F98/PT | - |
dc.rights | openAccess | eng |
dc.subject | Fermentation processes | eng |
dc.subject | Adaptive control | eng |
dc.subject | Model-based control | eng |
dc.subject | Sensors | eng |
dc.subject | Nonlinear systems | eng |
dc.subject | Lyapunov methods | eng |
dc.title | Model-based adaptive control of acetate concentration during the production of recombinant proteins with E. coli | eng |
dc.type | conferencePaper | eng |
dc.peerreviewed | yes | eng |
oaire.citationStartPage | 461 | por |
oaire.citationEndPage | 466 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 15 | por |
sdum.journal | IFAC proceedings volumes | por |
Aparece nas coleções: | CEB - Artigos em Livros de Atas / Papers in Proceedings |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Post-Print2003.pdf | 319,23 kB | Adobe PDF | Ver/Abrir |