Estimation of biomass concentration and multiple specific growth rates of fed-batch fermentation of recombinant E. coli

Abstract

In this paper, a two step estimation approach is proposed for estimation of multiple (specific) growth rates and biomass concentration of fed-batch fermentation of recombinant E. coli. It is considered the case when the process is described using a switch between two partial sub-models. On the first step two out of the three growth rates are estimated on the basis of observer-based estimators using on-line measurements of acetate concentration. In the second step, the third biomass growth rate and the biomass concentration, are estimated for both submodels, on the basis of estimation algorithms using on-line measurements of glucose concentration. The performances of the proposed estimation algorithms are investigated by simulations for a model of fed-batch fermentation of recombinant E. coli.