Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/30289
Título: | Liquid marbles for high-throughput biological screening of anchorage-dependent cells |
Autor(es): | Oliveira, N. M. Correia, Clara R. Reis, R. L. Mano, J. F. |
Palavras-chave: | High-throughput screening Hydrophobicity Image processing Lliquid marbles Microbioreactors Liquid marbles |
Data: | 2015 |
Editora: | Wiley-VCH Verlag |
Revista: | Advanced Healthcare Materials |
Citação: | 26. Oliveira, N. M., Correia, C. R., Reis, R. L., & Mano, J. F. (2015). Liquid Marbles for High-Throughput Biological Screening of Anchorage-Dependent Cells. Advanced Healthcare Materials, 4(2), 264-270. doi: 10.1002/adhm.201400310 |
Resumo(s): | Stable liquid marbles (LM) are produced by coating liquid droplets with a hydrophobic powder. The used hydrophobic powder is produced by fl uorosilanization of diatomaceous earth, used before to produce superhydrophobic structures. Here, the use of LM is proposed for high-throughput drug screening on anchorage-dependent cells. To provide the required cell adhesion sites inside the liquid environment of LM, surface-modifi ed poly( L -lactic acid) microparticles are used. A simple method that takes advantage from LM appealing features is presented, such as the ability to inject liquid on LM without disrupting (self-healing ability), and to monitor color changes inside of LM. After promoting cell adhesion, a cytotoxic screening test is performed as a proof of concept. Fe 3+ is used as a model cytotoxic agent and is injected on LM. After incubation, AlamarBlue reagent is injected and used to assess the presence of viable cells, by monitoring color change from blue to red. Color intensity is measured by image processing and the analysis of pictures takes using an ordinary digital camera. The proposed method is fully validated in counterpoint to an MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-te trazolium) colorimetric assay, a well-known method used for the cytotoxicity assessment. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/30289 |
DOI: | 10.1002/adhm.201400310 |
ISSN: | 2192-2640 |
Versão da editora: | http://onlinelibrary.wiley.com/doi/10.1002/adhm.201400310/abstract |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18095-adhm201400310.pdf Acesso restrito! | 838,9 kB | Adobe PDF | Ver/Abrir |