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https://hdl.handle.net/1822/31455
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Campo DC | Valor | Idioma |
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dc.contributor.author | Rodrigues, Priscila M. B. | por |
dc.contributor.author | Lima, Carolina A. | por |
dc.contributor.author | Filho, José Luiz L. | por |
dc.contributor.author | Teixeira, J. A. | por |
dc.contributor.author | Porto, Ana L. F. | por |
dc.contributor.author | Cunha, Maria G. Carneiro da | por |
dc.date.accessioned | 2014-12-01T15:04:47Z | - |
dc.date.available | 2014-12-01T15:04:47Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Rodrigues, Priscila M. B.; Lima, Carolina; Filho, J. L. Lima; Teixeira, José A.; Porto, A. L. F.; Carneiro-da-Cunha, M. G., Production and characterization of protease from Penicillium aurantiogriseum URM 4622. VALNATURA - A Europe-Latin América post-graduate research network in the VALorization of NATURAL resources, 2008. ISBN: 978-972-97810-5-6, 97-104 | - |
dc.identifier.isbn | 978-972-97810-5-6 | por |
dc.identifier.uri | https://hdl.handle.net/1822/31455 | - |
dc.description.abstract | Proteases with new properties are required due to their increasing industrial importance. In this work, the optimal fermentation conditions for the production of a protease from Penicillium aurantiogriseum dierchx (URM-4622) are presented together with partial characterization of the protease catalytic properties. The batch fermentation conditions that allow for the highest specific proteolytic activity are 26 ºC, pH 7.0, and 25 % saturation dissolved O2 concentration. The obtained protease is stable over a wide range of pH (5.8 to 9.5) and temperature (25 to 40 ºC) values. In the presence of Zn2+ a 26 % reduction in the enzyme proteolytic activity occurs and, in contrast, Mn2+ enhances its activity by 28.9 %. 96.2 % and 70.8 % of the protease activity are maintained after 90 min incubation in 5 and 10 % (v/v) H2O2 aqueous solutions, respectively. PMSF inhibition reveals that this enzyme is a serine protease. Protease is able to hydrolyze different proteins. | por |
dc.language.iso | eng | por |
dc.rights | openAccess | por |
dc.subject | Penicillium aurantiogriseum | por |
dc.subject | Production | por |
dc.subject | Characterization | por |
dc.subject | Protease | por |
dc.subject | Bacth | por |
dc.subject | Bioreactor | por |
dc.subject | Detergent | por |
dc.title | Production and characterization of protease from Penicillium aurantiogriseum URM 4622 | por |
dc.type | bookPart | - |
dc.comments | CEB17822 | por |
sdum.publicationstatus | published | - |
oaire.citationStartPage | 97 | por |
oaire.citationEndPage | 104 | por |
oaire.citationTitle | VALNATURA - A Europe-Latin América post-graduate research network in the VALorization of NATURAL resources | por |
dc.date.updated | 2014-11-28T17:53:07Z | - |
sdum.bookTitle | VALNATURA - A Europe-Latin América post-graduate research network in the VALorization of NATURAL resources | por |
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