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https://hdl.handle.net/1822/32471
Título: | Metabolic engineering of Saccharomyces cerevisiae ethanol strains PE-2 and CAT-1 for efficient lignocellulosic fermentation |
Autor(es): | Romaní, Aloia Pereira, Filipa Johansson, Björn Domingues, Lucília |
Palavras-chave: | Second generation bio-ethanol Xylose fermentation GRE3 aldose reductase Industrial Saccharomyces cerevisiae Simultaneous saccharification and co-fermentation (SSCF) |
Data: | 2015 |
Editora: | Elsevier B.V. |
Revista: | Bioresource technology |
Citação: | Romaní, Aloia; Pereira, Filipa; Johansson, Björn; Domingues, Lucília, Metabolic engineering of Saccharomyces cerevisiae ethanol strains PE-2 and CAT-1 for efficient lignocellulosic fermentation. Bioresource Technology, 179, 150-158, 2015. |
Resumo(s): | In this work, Saccharomyces cerevisiae strains PE-2 and CAT-1, commonly used in the Brazilian fuel ethanol industry, were engineered for xylose fermentation, where the first fermented xylose faster than the latter, but also produced considerable amounts of xylitol. An engineered PE-2 strain (MEC1121) efficiently consumed xylose in presence of inhibitors both in synthetic and corn-cob hydrolysates. Interestingly, the S. cerevisiae MEC1121 consumed xylose and glucose simultaneously, while a CEN.PK based strain consumed glucose and xylose sequentially. Deletion of the aldose reductase GRE3 lowered xylitol production to undetectable levels and increased xylose consumption rate which led to higher final ethanol concentrations. Fermentation of corn-cob hydrolysate using this strain, MEC1133, resulted in an ethanol yield of 0.47 g/g of total sugars which is 92% of the theoretical yield. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/32471 |
DOI: | 10.1016/j.biortech.2014.12.020 |
ISSN: | 0960-8524 |
e-ISSN: | 0960-8524 |
Versão da editora: | http://www.journals.elsevier.com/bioresource-technology/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_19525_1.pdf | 559,69 kB | Adobe PDF | Ver/Abrir |