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https://hdl.handle.net/1822/33282
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Campo DC | Valor | Idioma |
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dc.contributor.author | Sousa, Cátia A. | por |
dc.contributor.author | Hanselaer, Simon | por |
dc.contributor.author | Soares, Eduardo V. | por |
dc.date.accessioned | 2015-01-27T19:36:18Z | - |
dc.date.available | 2015-01-27T19:36:18Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Sousa, Cátia A.; Hanselaer, Simon; Soares, Eduardo V., ABCC subfamily vacuolar transporters are involved in Pb (Lead) detoxification in Saccharomyces cerevisiae. Applied Biochemistry and Biotechnology, 175(1), 65-74, 2015 | por |
dc.identifier.issn | 0273-2289 | por |
dc.identifier.uri | https://hdl.handle.net/1822/33282 | - |
dc.description.abstract | The present work has as objective to contribute for the elucidation of the mechanism associated with Pb detoxification, using the yeast Saccharomyces cerevisiae as a model organism. The deletion of GTT1 or GTT2 genes, coding for functional glutathione transferases (GST) enzymes in S. cerevisiae, caused an increased susceptibility to high Pb concentrations (5001000 mol L1). These results suggest that the formation of glutathione-Pb conjugate (GS-Pb), dependent of GSTs, is important in Pb detoxification. The involvement of ATP-binding cassette (ABC) vacuolar transporters, belonging to class C subfamily (ABCC) in vacuolar compartmentalization of Pb, was evaluated. For this purpose, mutant strains disrupted in YCF1, VMR1, YBT1 or BPT 1 genes were used. All mutants tested, without vacuolar ABCC transporters, presented an increased sensitivity to 5001000 mol L1 Pb comparative to wild-type strain. Taken together, the obtained results suggest that Pb detoxification, by vacuolar compartmentalization, can occur as a result of the concerted action of GSTs and vacuolar ABCC transporters. Pb is conjugated with glutathione, catalysed by glutathione transferases and followed to the transport of GS-Pb conjugate to the vacuole by ABCC transporters. | por |
dc.description.sponsorship | The authors thank the FCT Strategic Project PEst-OE/EQB/LA0023/2013. | por |
dc.language.iso | eng | por |
dc.publisher | Springer | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0023%2F2013/PT | - |
dc.rights | openAccess | por |
dc.subject | Glutathione transferases (GSTs) | por |
dc.subject | Heavy metal | por |
dc.subject | Lead (Pb) | por |
dc.subject | Vacuolar ABC transporters | por |
dc.subject | Yeast | por |
dc.title | ABCC subfamily vacuolar transporters are involved in Pb (lead) detoxification in Saccharomyces cerevisiae | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.springer.com/chemistry/biotechnology/journal/12010 | por |
dc.comments | CEB18168 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 65 | por |
oaire.citationEndPage | 74 | por |
oaire.citationIssue | 1 | por |
oaire.citationConferencePlace | United States | - |
oaire.citationTitle | Applied Biochemistry and Biotechnology | por |
oaire.citationVolume | 175 | por |
dc.date.updated | 2015-01-27T18:58:58Z | - |
dc.identifier.eissn | 0273-2289 | - |
dc.identifier.doi | 10.1007/s12010-014-1252-0 | por |
dc.identifier.pmid | 25240850 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Applied Biochemistry and Biotechnology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
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document_18168_1.pdf | 559,81 kB | Adobe PDF | Ver/Abrir |